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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Peptide fingerprinting of the neurotoxic fractions isolated from the secretions of sea anemones Stichodactyla helianthus and Bunodosoma granulifera. New members of the APETx-like family identified by a 454 pyrosequencing approach

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Autor(es):
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Alexei Rodriguez, Armando [1] ; Cassoli, Juliana Silva [2] ; Sa, Fei [3] ; Dong, Zhi Qiang [3] ; de Freitas, Jose Carlos [4] ; Pimenta, Adriano M. C. [2] ; de Lima, Maria Elena [2] ; Konnoe, Katsuhiro [5] ; Lee, Simon Ming Yuen [3] ; Garateix, Anoland [1] ; Zaharenko, Andre J. [6]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Ctr Bioprod Marinos Cebimar, Nuevo Vedado 10400, Habana - Cuba
[2] Univ Fed Minas Gerais UFMG, Lab Venenos & Toxinas Animais, Belo Horizonte, MG - Brazil
[3] Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Macao - Peoples R China
[4] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Sao Paulo - Brazil
[5] Toyama Univ, Inst Nat Med, Toyama 930 - Japan
[6] InsButantan, Lab Genet, BR-05503900 Sao Paulo - Brazil
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: Peptides; v. 34, n. 1, SI, p. 26-38, MAR 2012.
Citações Web of Science: 30
Resumo

Sea anemones are known to contain a wide diversity of biologically active peptides, mostly unexplored according to recent peptidomic and transcriptomic studies. In the present work, the neurotoxic fractions from the exudates of Stichodactyla helianthus and Bunodosoma granulifera were analyzed by reversed-phase chromatography and mass spectrometry. The first peptide fingerprints of these sea anemones were assessed, revealing the largest number of peptide components (156) so far found in sea anemone species, as well as the richer peptide diversity of B. granulifera in relation to S. helianthus. The transcriptomic analysis of B. granulifera, performed by massive cDNA sequencing with 454 pyrosequencing approach allowed the discovery of five new APETx-like peptides (U-AITX-Bg1a-e - including the full sequences of their precursors for four of them), which together with type 1 sea anemone sodium channel toxins constitute a very distinguishable feature of studied sea anemone species belonging to genus Bunodosoma. The molecular modeling of these new APETx-like peptides showed a distribution of positively charged and aromatic residues in putative contact surfaces as observed in other animal toxins. On the other hand, they also showed variable electrostatic potentials, thus suggesting a docking onto their targeted channels in different spatial orientations. Moreover several crab paralyzing toxins (other than U-AITX-Bg1a-e), which induce a variety of symptoms in crabs, were isolated. Some of them presumably belong to new classes of crab-paralyzing peptide toxins, especially those with molecular masses below 2 kDa, which represent the smallest peptide toxins found in sea anemones. (C) 2011 Elsevier Inc. All rights reserved. (AU)

Processo FAPESP: 07/56525-3 - Construção de uma biblioteca de cDNA dos tentáculos da anêmona do mar Bunodosoma cangicum e clonagem de neurotoxinas
Beneficiário:Andre Junqueira Zaharenko
Linha de fomento: Bolsas no Brasil - Pós-Doutorado