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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Antimicrobial Activity of Essential Oils against Streptococcus mutans and their Antiproliferative Effects

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Autor(es):
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de Carvalho Galvao, Livia Camara [1] ; Furletti, Vivian Fernandes [2] ; Fernandes Bersan, Salete Meyre [1] ; da Cunha, Marcos Guilherme [1] ; Tasca Gois Ruiz, Ana Lucia [2] ; de Carvalho, Joao Ernesto [2] ; Sartoratto, Adilson [2] ; Garcia Rehder, Vera Lucia [2] ; Figueira, Glyn Mara [2] ; Teixeira Duarte, Marta Cristina [2] ; Ikegaki, Masarahu [3] ; de Alencar, Severino Matias [4] ; Rosalen, Pedro Luiz [1]
Número total de Autores: 13
Afiliação do(s) autor(es):
[1] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Pharmacol Anesthesiol & Therapeut, BR-13414903 Piracicaba, SP - Brazil
[2] Univ Campinas UNICAMP, Res Ctr Chem Biol & Agr, BR-13083970 Campinas, SP - Brazil
[3] Univ Fed Alfenas, Sch Pharm & Dent, BR-37130000 Alfenas, MG - Brazil
[4] Univ Sao Paulo, ESALQ, Dept Agrifood Ind Food & Nutr, BR-13418900 Piracicaba, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Evidence-based Complementary and Alternative Medicine; 2012.
Citações Web of Science: 28
Resumo

This study aimed to evaluate the activity of essential oils (EOs) against Streptococcus mutans biofilm by chemically characterizing their fractions responsible for biological and antiproliferative activity. Twenty EO were obtained by hydrodistillation and submitted to the antimicrobial assay (minimum inhibitory (MIC) and bactericidal (MBC) concentrations) against S. mutans UA159. Thin-layer chromatography and gas chromatography/mass spectrometry were used for phytochemical analyses. EOs were selected according to predetermined criteria and fractionated using dry column; the resulting fractions were assessed by MIC and MBC, selected as active fractions, and evaluated against S. mutans biofilm. Biofilms formed were examined using scanning electron microscopy. Selected EOs and their selected active fractions were evaluated for their antiproliferative activity against keratinocytes and seven human tumor cell lines. MIC and MBC values obtained for EO and their active fractions showed strong antimicrobial activity. Chemical analyses mainly showed the presence of terpenes. The selected active fractions inhibited S. mutans biofilm formation (P < 0.05) did not affect glycolytic pH drop and were inactive against keratinocytes, normal cell line. In conclusion, EO showed activity at low concentrations, and their selected active fractions were also effective against biofilm formed by S. mutans and human tumor cell lines. (AU)

Processo FAPESP: 11/14757-0 - Avaliação da atividade antimicrobiana de óleos essenciais sobre patógenos bucais
Beneficiário:Pedro Luiz Rosalen
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 09/12353-0 - Avaliação da atividade antimicrobiana de óleos essenciais de plantas contra microrganismos do grupo mutans
Beneficiário:Lívia Câmara de Carvalho Galvão
Linha de fomento: Bolsas no Brasil - Mestrado