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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

HEV infection in swine from Eastern Brazilian Amazon: Evidence of co-infection by different subtypes

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Autor(es):
Souza de Souza, Alex Junior [1, 2] ; Gomes-Gouvea, Michele Soares [3, 4] ; Pereira Soares, Manoel do Carmo [1] ; Rebello Pinho, Joao Renato [3, 4] ; Malheiros, Andreza Pinheiro [1] ; Carneiro, Liliane Almeida [1] ; Lopes dos Santos, Debora Regina [5] ; Assuncao Pereira, Washington Luiz [6]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Inst Evandro Chagas, Secao Hepatol, BR-66093020 Belem, PA - Brazil
[2] Fac Integradas Tapajos, Ctr Ciencias Biol & Saude, BR-68010200 Santarem, PA - Brazil
[3] Univ Sao Paulo, Sch Med, Sao Paulo Inst Trop Med, Lab Gastroenterol & Hepatol, BR-05508 Sao Paulo - Brazil
[4] Univ Sao Paulo, Sch Med, Dept Gastroenterol, BR-05508 Sao Paulo - Brazil
[5] Univ Fed Rural Rio de Janeiro, Inst Vet, BR-23890000 Rio De Janeiro, RJ - Brazil
[6] Univ Fed Rural Amazonia, Inst Saude & Prod Anim, BR-66077901 Belem, PA - Brazil
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES; v. 35, n. 5, p. 477-485, SEP 2012.
Citações Web of Science: 24
Resumo

Hepatitis E virus (HEV) is a fecal-orally transmitted member of the genus Hepevirus that causes acute hepatitis in humans and is widely distributed throughout the world. Pigs have been reported as the main source of genotypes 3 and 4 infection to humans in non-endemic areas. To investigate HEV infection in pigs from different regions of Para state (Eastern Brazilian Amazon), we performed serological and molecular analyses of serum, fecal and liver samples from 151 adult pigs slaughtered between April and October 2010 in slaughterhouses in the metropolitan region of Belem, Para. Among the animals tested, 8.6% (13/151) were positive for anti-HEV IgG but not for anti-HEV IgM. HEV RNA was detected in 4.8% (22/453) of the samples analyzed and 9.9% (15/151) of the animals had at least one positive sample. Phylogenetic analysis showed that all sequences belonged to genotype 3 that were related to human isolates from other non-endemic regions, suggesting that the isolates had zoonotic potential. Subtypes 3c and 3f were simultaneously detected in some pigs, suggesting co-infection by more than one strain and/or the presence of a recombinant virus. These results constitute the first molecular and serologic evidence of swine HEV circulation in the Eastern Brazilian Amazon. (C) 2012 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 09/53946-3 - EMU: aquisição de analisador genético de DNA para o sequenciamento automático de DNA
Beneficiário:João Renato Rebello Pinho
Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
Processo FAPESP: 10/50081-9 - Prevalência de resistência primária aos antivirais utilizados no tratamento da hepatite B entre pacientes com infecção crônica pelo vírus da hepatite B não submetidos a tratamento
Beneficiário:Michele Soares Gomes Gouvêa
Linha de fomento: Bolsas no Brasil - Doutorado