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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Proteome analysis of spinal cord during the clinical course of monophasic experimental autoimmune encephalomyelitis

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Autor(es):
Farias, Alessandro S. [1, 2] ; Martins-de-Souza, Daniel [3, 4, 5] ; Guimaraes, Leandro [3] ; Pradella, Fernando [2] ; Moraes, Adriel S. [2] ; Facchini, Gustavo ; Novello, Jose Camillo [3] ; Santos, Leonilda M. B.
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Neuroimmunol Unit, Dept Genet Evolut & Bioagents, Inst Biol, Campinas, SP - Brazil
[2] Univ Estadual Campinas, Neuroimmunomodulat Young Investigator Grp, Dept Genet Evolut & Bioagents, Campinas, SP - Brazil
[3] Univ Estadual Campinas, Dept Biochem, Lab Prote, Campinas, SP - Brazil
[4] Max Planck Inst Psychiat Prote & Biomarkers, Munich - Germany
[5] Univ Sao Paulo, Fac Med, Inst Psychiat, Lab Neurosci LIM 27, BR-05508 Sao Paulo - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: PROTEOMICS; v. 12, n. 17, SI, p. 2656-2662, AUG 2012.
Citações Web of Science: 10
Resumo

The induction of autoimmune encephalomyelitis (EAE) in Lewis rats results in a period of exacerbation followed by complete recovery. Therefore, this model is widely used for studying the evolution of multiple sclerosis. In the present investigation, differentially expressed proteins in the spinal cord of Lewis rats during the evolution of EAE were assessed using the combination of 2DE and MALDI-TOF MS. The majority of the differentially expressed proteins were identified during the acute phase of EAE, in relation to naive control animals. On the other hand, recovered rats presented a similar protein expression pattern in comparison with the naive ones. This observation can be explained, at least in part, by the intense catabolism existent in acute phase due to nervous tissue damage. In recovered rats, we have described the upregulation of proteins that are apparently involved in the recovery of damaged tissue, such as light and medium neurofilaments, glial fibrillary acidic protein, tubulins subunits, and quaking protein. These proteins are involved mainly in cell growth, myelination, and remyelination as well as in astrocyte and oligodendrocyte maturation. The present study has demonstrated that the inflammatory response, characterized by an increase of the proliferative response and infiltration of autoreactive T lymphocytes in the central nervous system, occurs simultaneously with neurodegeneration. (AU)

Processo FAPESP: 09/15620-9 - Análise do padrão migratório e efetor das células encefalitogênicas e reguladoras durante a evolução clínica da encefalomielite experimental autoimune induzida em ratos Lewis e camundongos C57BL/6 (WT e IFN³(KO)
Beneficiário:Alessandro dos Santos Farias
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 11/18728-5 - Estudo do padrão migratório, efetor e regulador dos Linfócitos T auto-reativos, previamente transduzidos com GFP, nas doenças desmielinizantes experimentais
Beneficiário:Alessandro dos Santos Farias
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 09/18227-6 - Estudo dos mecanismos imunológicos efetores e reguladores nas doenças desmielinizantes humanas e experimentais
Beneficiário:Leonilda Maria Barbosa dos Santos
Linha de fomento: Auxílio à Pesquisa - Regular