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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

In vitro study of antiviral activity of mycophenolic acid on Brazilian Orthobunyaviruses

Texto completo
Autor(es):
Livonesi, Márcia Cristina ; Sousa, Ricardo Luiz Moro de ; Figueiredo, Luiz Tadeu Moraes [3]
Número total de Autores: 3
Tipo de documento: Artigo Científico
Fonte: Intervirology; v. 50, n. 3, p. 204-208, Apr. 2007.
Área do conhecimento: Ciências Biológicas - Microbiologia
Assunto(s):Ácido micofenólico   Vírus de RNA   Bunyaviridae   Orthobunyavirus   Antivirais
Resumo

Oropouche, Caraparu, Guama, Guaroa and Tacaiuma are ssRNA viruses that belong to the genus Orthobunyavirus and have been associated with human febrile illnesses and/or encephalitis. In this study, we evaluated the antiviral action of mycophenolic acid (MPA) on theseorthobunyaviruses to achieve a therapeutic agent to treat the diseases caused by these viruses. The in vitro antiviral evaluation to MPA was done by using plaque assay at different periods of treatment. Results showed that MPA at a concentration of 10 ug/ml has significant antiviral activity on Tacaiuma virus when treatment was initiated either 24 h before or 2 h after viral infection. Moreover, MPA has an inhibitory effect on Guama virus replication, but only when treatment was initiated before cell infection. Addition of guanosine in the culture reverted the inhibitory effect of MPA on Tacaiuma and Guama viruses, suggesting that the antiviral activity of this substance was via depletion of the intracellular guanosine pool. Our results suggest that MPA would not be a good therapeutic agent to treat the diseases caused by Oropouche, Caraparu, Guama, Guaroa, and Tacaiuma viruses. (AU)

Processo FAPESP: 03/03682-3 - Estudos sobre arboviroses e viroses associadas a roedores
Beneficiário:Luiz Tadeu Moraes Figueiredo
Linha de fomento: Auxílio à Pesquisa - Temático