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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Intestinal Lymph-Borne Factors Induce Lung Release of Inflammatory Mediators and Expression of Adhesion Molecules After an Intestinal Ischemic Insult

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Autor(es):
Breithaupt-Faloppa, Ana Cristina ; Vitoretti, Luana Beatriz ; Cavriani, Gabriela ; Lino-dos-Santos-Franco, Adriana ; Sudo-Hayashi, Lia Siguemi ; Oliveira-Filho, Ricardo Martins ; Boris Vargaftig, B. ; Tavares-de-Lima, Wothan [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Ciencias Biomed, Lab Fisiopatol Inflamacao Expt, Dept Pharmacol, BR-05508900 Sao Paulo - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF SURGICAL RESEARCH; v. 176, n. 1, p. 195-201, JUL 2012.
Citações Web of Science: 9
Resumo

Background. Intestinal ischemia and reperfusion (I/R) is a documented cause of acute lung injury (ALI) and systemic inflammation. We previously reported that obstruction of thoracic lymphatic flow during intestinal I/R blunts pulmonary neutrophil recruitment and microvascular injury and decreases the systemic levels of tumor necrosis factor. Here, we consider the existence of a gut-lung axis promoting the induction of systemic inflammation, whereby drained intestinal lymph stimulates lung expression of adhesion molecules and matrix components and generation of inflammatory mediators. Material and Methods. Upon administration of anesthesia, male Wistar rats were subjected to occlusion of the superior mesenteric artery for 45 min, followed by 2 h of intestinal reperfusion (I/R); groups of rats were subjected to I/R with or without thoracic lymphatic duct ligation immediately before the procedure. The non-manipulated rats were used to investigate basal parameters. Results. Obstruction of thoracic lymphatic flow before intestinal I/R decreased the ability of cultured lung tissue explants to release IL-1 beta, IL-10, and VEGF. In contrast, lymphatic obstruction normalized the elevated lung expression of PECAM-1 caused by intestinal I/R. On the other hand, lung E-selectin expression was significantly reduced, whereas fibronectin expression and collagen synthesis were not affected. Lymph levels of LTB4 and TXB2 were found to be significantly increased. Conclusions. These data suggest that lymph factors drained from the intestine during ischemic trauma stimulate the lung to generate inflammatory mediators and alter the expression of adhesion molecules. Disturbances in lung homeostasis mediated by lymph might contribute to the spread of inflammatory processes, thereby accounting for the systemic inflammation induced by intestinal I/R. (C) 2012 Elsevier Inc. All rights reserved. (AU)

Processo FAPESP: 07/07139-3 - Investigando o papel da heme-oxigenase 1 em diferentes processos inflamatórios renais em modelos animais
Beneficiário:Niels Olsen Saraiva Câmara
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 05/02271-5 - Estudo ex-vivo dos mecanismos reguladores da interação leucócito-endotélio após trauma esplâncnico e sua relevância para a inflamação pulmonar: envolvimento do sistema linfático
Beneficiário:Wothan Tavares de Lima
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 09/54823-2 - Avaliação do papel dos hormônios sexuais femininos e do sistema linfático no controle da inflamação pulmonar e sistêmica após a isquêmica e reperfusão intestinal em ratas
Beneficiário:Wothan Tavares de Lima
Linha de fomento: Auxílio à Pesquisa - Regular