Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Genotypic Characterization of Cryptosporidium hominis from Water Samples in Sao Paulo, Brazil

Texto completo
Autor(es):
Araujo, Ronalda S. [1] ; Dropa, Milena [1] ; Fernandes, Licia N. [1] ; Carvalho, Terezinha T. [1] ; Sato, Maria Ines Z. [1] ; Soares, Rodrigo M. [1] ; Matte, Glavur R. [1] ; Matte, Maria Helena [2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, BR-01246904 Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Publ Hlth, BR-01246904 Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: American Journal of Tropical Medicine and Hygiene; v. 85, n. 5, p. 834-838, NOV 2011.
Citações Web of Science: 8
Resumo

The protozoan parasite Cryptosporidium has emerged as one of the most important water contaminants, causing waterborne outbreaks of diarrheal diseases worldwide. The small size of oocysts under the microscope and the possibility of changes in characteristics of oocysts, mainly in environmental samples, make the taxonomy of the genus difficult if morphologic characteristics are considered. This limitation encouraged the application of molecular methods to identify this microorganism. The aim of this study was to detect and identify by nested-polymerase chain reaction oocysts of Cryptosporidium present in water samples in the state of Sao Paulo, Brazil. Water samples were concentrated through a membrane filter, DNA was extracted by using a standard technique, and both amplification reactions used forward and reverse oligonucleotides that were complementary to Cryptosporidium 18S ribosomal RNA gene sequences. Thirty water samples from different sites of collection in the state of Sao Paulo were evaluated. Cryptosporidium oocysts were detected in 30% of the samples. By genoptyping, C. hominis and Cryptosporidium sp. were identified in recreational water and C meleagridis was identified in surface water samples. This is the first report of C. hominis in environmental samples in Brazil. Although identification of Cryptosporidium is still a difficult task, molecular methods are essential for specific identification and are a helpful tool to aid to understand the epidemiology of this parasite in Brazil. (AU)

Processo FAPESP: 06/01532-2 - Genotipagem de Cryptosporidium spp. provenientes de amostras ambientais como fonte de informação da dispersão de espécies no ambiente
Beneficiário:Ronalda Silva de Araújo
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 05/03783-0 - Genotipagem de Cryptosporidium spp. provenientes de amostras clínicas e ambientais como fonte de informação da dispersão de espécies no ambiente e na clínica
Beneficiário:Maria Helena Matte
Linha de fomento: Auxílio à Pesquisa - Regular