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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Detection of Human Bocavirus mRNA in Respiratory Secretions Correlates with High Viral Load and Concurrent Diarrhea

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Autor(es):
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Proenca-Modena, Jose Luiz [1, 2] ; Gagliardi, Talita Bianca [1, 2] ; de Paula, Flavia Escremim [1, 2] ; Iwamoto, Marisa Akiko [2] ; Criado, Miria Ferreira [1, 2] ; Camara, Ataide A. [3] ; Acrani, Gustavo Olszanski [1, 2] ; Leite Cintra, Otavio Augusto [4] ; Cervi, Maria Celia [4] ; de Paula Arruda, Luisa Karla [5] ; Arruda, Eurico [1, 2]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Med, Dept Cell Biol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Med, Virol Res Ctr, Sao Paulo - Brazil
[3] Santa Lydia Hosp, Sao Paulo - Brazil
[4] Univ Sao Paulo, Sch Med, Dept Pediat, Sao Paulo - Brazil
[5] Univ Sao Paulo, Sch Med, Dept Internal Med, Sao Paulo - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 6, n. 6 JUN 20 2011.
Citações Web of Science: 29
Resumo

Human bocavirus (HBoV) is a parvovirus recently identified in association with acute respiratory infections (ARI). Despite its worldwide occurrence, little is known on the pathogenesis of HBoV infections. In addition, few systematic studies of HBoV in ARI have been conducted in Latin America. Therefore, in order to test whether active viral replication of human bocavirus is associated with respiratory diseases and to understand the clinical impact of this virus in patients with these diseases, we performed a 3-year retrospective hospital-based study of HBoV in outpatients and inpatients with symptoms of Acute Respiratory Infections (ARI) in Brazil. Nasopharyngeal aspirates (NPAs) from 1015 patients with respiratory symptoms were tested for HBoV DNA by PCR. All samples positive for HBoV were tested by PCR for all other respiratory viruses, had HBoV viral loads determined by quantitative real time PCR and, when possible, were tested by RT-PCR for HBoV VP1 mRNA, as evidence of active viral replication. HBoV was detected in 4.8% of patients, with annual rates of 10.0%, 3.0% and 3.0% in 2005, 2006 and 2007, respectively. The range of respiratory symptoms was similar between HBoV-positive and HBoV-negative ARI patients. However, a higher rate of diarrhea was observed in HBoV-positive patients. High HBoV viral loads (> 10(8) copies/mL) and diarrhea were significantly more frequent in patients with exclusive infection by HBoV and in patients with detection of HBoV VP1 mRNA than in patients with viral co-infection, detected in 72.9% of patients with HBoV. In summary, our data demonstrated that active HBoV replication was detected in a small percentage of patients with ARI and was correlated with concurrent diarrhea and lack of other viral co-infections. (AU)

Processo FAPESP: 09/51818-8 - O perfil das infecções virais em patologias crônicas do trato respiratório superior: hipertrofia adenoamigdaliana, otite média secretora e rinossinusite
Beneficiário:Wilma Terezinha Anselmo-Lima
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 08/50617-6 - Estudos sobre vírus emergentes incluindo arbovirus, robovirus, vírus respiratórios e de transmissão congênita, no Centro de Pesquisa em Virologia da Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo
Beneficiário:Luiz Tadeu Moraes Figueiredo
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 09/52388-7 - Papel de Bocavirus humano na patogênese de adenoamigdalites crônicas
Beneficiário:José Luiz Proença Módena
Linha de fomento: Bolsas no Brasil - Pós-Doutorado