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(Referência obtida automaticamente do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Interleukin-5 mediates peritoneal eosinophilia induced by the F1 cell wall fraction of Histoplasma capsulatum

Texto completo
Autor(es):
Sá-Nunes, A. ; Medeiros, A. I. ; Faccioli, L. H. [3]
Número total de Autores: 3
Tipo de documento: Artigo Científico
Fonte: Brazilian Journal of Medical and Biological Research; v. 37, n. 3, p. 343-346, Mar. 2004.
Área do conhecimento: Ciências Biológicas - Imunologia
Notas: O artigo da revista consta como produto de dois Processos: 95/09691-6 e 02/12856-2 em nome de Lúcia Helena Faccioli
Assunto(s):Interleucina-5   Sistemas sanguíneo e imune   Eosinófilos   Fungos mitospóricos   Histoplasma
Resumo

An alkali-insoluble fraction 1 (F1), which contains mainly BETA-glucan isolated from the cell wall of Histoplasma capsulatum, induces eosinophil recruitment into the peritoneal cavity of mice. The present study was carried out to determine the participation of interleukin-5 (IL-5) in this process. Inbred C57BL/6 male mice weighing 15-20 g were treated ip with 100 µg of anti-IL-5 monoclonal antibody (TRFK-5, N = 7) or an isotype-matched antibody (N = 7), followed by 300 µg F1 in 1 ml PBS ip 24 h later. Controls (N = 5) received only 1 ml PBS. Two days later, cells from the peritoneal cavity were harvested by injection of 3 ml PBS and total cell counts were determined using diluting fluid in a Neubauer chamber. Differential counts were performed using Rosenfeld-stained cytospin preparations. The F1 injection induced significant (P < 0.01) leukocyte recruitment into the peritoneal cavity (8.4 x 106 cells/ml) when compared with PBS alone (5.5 x 106 cells/ml). Moreover, F1 selectively (P < 0.01) induced eosinophil recruitment (1 x 106 cells/ml) when compared to the control group (0.07 x 106 cells/ml). Treatment with TRFK-5 significantly (P < 0.01) inhibited eosinophil recruitment (0.18 x 106 cells/ml) by F1 without affecting recruitment of mononuclear cells or neutrophils. We conclude that the F1 fraction of the cell wall of H. capsulatum induces peritoneal eosinophilia by an IL-5-dependent mechanism. Depletion of this cytokine does not have effect on the recruitment of other cell types induced by F1. (AU)

Processo FAPESP: 02/12856-2 - Modulação das respostas imunes inata e adquirida por leucotrienos e prostaglandinas
Beneficiário:Lúcia Helena Faccioli
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 95/09691-6 - Mecanismos envolvidos no recrutamento de eosinófilos e sua participação no desenvolvimento da hiper-reatividade brônquica
Beneficiário:Lúcia Helena Faccioli
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores