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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Phenotypic and molecular characterization of recent and archived Erysipelothrix spp. isolated from Brazilian swine

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Autor(es):
Coutinho, Tania A. [1] ; Imada, Yumiko [2] ; Barcellos, David E. S. N. [3] ; Oliveira, Sergio J. [4] ; Moreno, Andrea M. [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, FMVZ, Lab Sanidade Suina & Virol, BR-05508270 Sao Paulo - Brazil
[2] Natl Inst Anim Hlth, Dept Biol Prod, Tsukuba, Ibaraki 305 - Japan
[3] Univ Fed Rio Grande do Sul, Fac Vet FAVET, Setor Suinos, Porto Alegre, RS - Brazil
[4] Univ Luterana Brasil ULBRA, Fac Vet, Lab Microbiol Clin & Alimentos, Canoas - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE; v. 69, n. 2, p. 123-129, FEB 2011.
Citações Web of Science: 6
Resumo

One hundred fifty-one Erysipelothrix spp. isolates from Brazilian swine were characterized by serotyping, determination of antimicrobial susceptibility, amplified fragment length polymorphism (AFLP), and pulsed-field gel electrophoresis (PFGE). Among all isolates, 139 were classified in 18 different serotypes and serotype 2b was the most frequent. The susceptibility profiles of the isolates were very similar among each other, which did not permit subtyping Erysipelothrix spp. isolates by the antimicrobial susceptibility testing. Despite the fact that AFLP and PFGE provided the same discriminatory index (0.98), PFGE was more discriminatory than AFLP, given the types of groups it generates. Regardless the technique employed (AFLP or PFGE), no discrimination between recent and historical isolates was established, neither a fixed epidemiologic pattern for their grouping was observed. Nevertheless, AFLP could be an interesting alternative for discriminating the Erysipelothrix species, while PFGE could be an indication for discerning this bacterium according to the serotypes. (C) 2011 Elsevier Inc. All rights reserved. (AU)

Processo FAPESP: 06/55502-7 - Caracterização de amostras de Erysipelothrix spp isoladas de suínos nos últimos trinta anos
Beneficiário:Tania Alen Coutinho
Linha de fomento: Bolsas no Brasil - Doutorado