Immunohistochemical detection of metalloproteinase-9 (MMP-9), anti-oxidant like 1 protein (AOP-1) and synaptosomal-associated protein (SNAP-25) in the cerebella of dogs naturally infected with spontaneous canine distemper

Autor(es)

Bregano, Livia C. ^[1] ; Agostinho, Sabrina D. ^[1] ; Roncatti, Flavio L. B. T. ^[1] ; Pires, Marcilia C. ^[1] ; Riva, Henrique G. ^[1] ; Luvizotto, Maria C. R. ^{[1, 2]} ; Cardoso, Tereza C. ^[1]

Afiliação do(s) autor(es)

^[1] Fac Med Vet, Virol Lab, Sao Paulo - Brazil

^[2] Fac Med Vet, Lab Anim Pathol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FOLIA HISTOCHEMICA ET CYTOBIOLOGICA; v. 49, n. 1, p. 41-48, 2011.
• Citações Web of Science: 3

Resumo

In most viral infections of the central nervous system (CNS), the integrity of brain extracelluar matrix (ECM), oxidative stress and dysfunction in neuronal transmission may contribute to the observed pathology. The purpose of this study was to investigate the role of these factors in demyelinating canine distemper virus (CDV) infections. Regardless of ECM integrity, the expression of metalloproteinase-9 (MMP-9) was visualized in microglial-like cells, whereas the expression of anti-oxidant like-1 (AOP-1) and synaptosomal associated protein (SNAP-25) was frequently detected in Purkinje cells (r(2) = 0.989; p < 0.05), regardless of whether the lesions were classified as acute or chronic. Increased numbers of immunolabeled microglia-like cells and reactive gliosis were observed in advanced cases of demyelinating CDV, suggesting that the expression of AOP-1 and SNAP-25 is correlated with the ultimate death of affected cells. Our findings bring a new perspective to understanding the role of the AOP-1, MMP-9 and SNAP-25 proteins in mediating chronic leukoencephalitis caused by CDV. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 1, pp. 41-48) (AU)

• Processo FAPESP: 08/03802-2 - Aspectos da neuropatogênese da infecção aguda pelo herpesvírus tipo 5 em encéfalos bovinos: expressão da proteína glial fibrilar ácida (GFAP), vimentina (VIM), metallothionesina, proteínas associadas a sinapse e apoptose celular pela técnica de imunohistoquímica
• Beneficiário: Tereza Cristina Cardoso da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular