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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Characterization of the C-terminal half of human juvenile myoclonic epilepsy protein EFHC1: Dimer formation blocks Ca2+ and Mg2+ binding to its functional EF-hand

Texto completo
Autor(es):
Murai, Marcelo J. [1] ; Sassonia, Rogerio C. [2] ; Zamboni, Andre H. [1] ; Conte, Fabio F. [1] ; Martins-de-Souza, Daniel [3] ; Aparicio, Ricardo [2] ; de Oliveira, Marcelo G. [2] ; Lopes-Cendes, Iscia [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Fac Med Sci, Dept Med Genet, BR-13083970 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Inst Chem, BR-13083970 Campinas, SP - Brazil
[3] Univ Estadual Campinas, Inst Biol, Dept Biochem, BR-13083970 Campinas, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Archives of Biochemistry and Biophysics; v. 477, n. 1, p. 131-138, SEP 1 2008.
Citações Web of Science: 5
Resumo

Human EFHC1 is a member of the EF-hand superfamily of Ca2+-binding proteins with three DM10 domains of unclear function. Point mutations in the EFHC1 gene are related to juvenile myoclonic epilepsy, a fairly common idiopathic generalized epilepsy. Here, we report the first structural and thermodynamic analyses of the EFHC1C-terminus (residues 403-640; named EFHC1C), comprising the last DM10 domain and the EF-hand motif. Circular dichroism spectroscopy revealed that the secondary structure of EFHC1C is composed by 34% of alpha helices and 17% of beta -strands. Size exclusion chromatography and mass spectrometry showed that under oxidizing condition EFHC1C dimerizes through the formation of disulfide bond. Tandem mass spectrometry (MS/MS) analysis of peptides generated by trypsin digestion suggests that the Cys575 is involved in intermolecular S-S bond. In addition, DTNB assay showed that each reduced EFHC1C molecule has one accessible free thiol. Isothermal titration calorimetry (ITC) showed that while the interaction between Ca2+ and EFHC1C is enthalpically driven (Delta H= -58.6 to -67 kj/mol and T Delta S = -22.5 to -31 kj/mol) the interaction between Mg2+ and EFHC1 C involves an entropic gain,and is similar to 5 times less enthalpically favorable (Delta H= -11.7 to -14kj/mol and T Delta S=21.9 to 19kj/mol) than for Ca2+ binding. It was also found that under reducing condition Ca2+ or Mg2+ ions bind to EFH1C in a1/1 molar ratio, while under oxidizing condition this ratio is reduced, showing that EFH1C dimerization block Ca2+ and Mg2+ binding. (AU)

Processo FAPESP: 05/03234-6 - Estudos estruturais da LGI1 humana
Beneficiário:Ricardo Aparicio
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 04/03600-0 - Estudos funcionais do gene LGI1 no contexto da epilepsia de lobo temporal lateral
Beneficiário:Iscia Teresinha Lopes Cendes
Linha de fomento: Auxílio à Pesquisa - Regular