Effect of combined treatment of hydrolysis and polymerization with transglutaminase on beta-lactoglobulin antigenicity

Autor(es)

Sabadin, Isabele Serimarco ^[1] ; Villas-Boas, Mariana Battaglin ^[1] ; Zollner, Ricardo de Lima ^[2] ; Netto, Flavia Maria ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Food Engn, Dept Food & Nutr, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Dept Internal Med, Lab Immunol & Expt Allergy, Fac Med Sci, Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Fac Engn Alimentos, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: EUROPEAN FOOD RESEARCH AND TECHNOLOGY; v. 235, n. 5, p. 801-809, NOV 2012.
• Citações Web of Science: 15

Resumo

The effect of combined treatments of hydrolysis with different proteases, and subsequent polymerization with transglutaminase on the antigenic activity of beta-Lg was studied. For the hydrolysis of beta-Lg using Alcalase, Neutrase or bromelain, the reaction conditions were 3 % beta-Lg and enzyme:substrate 25 U g(-1) of protein, as was defined using factorial study. Under these conditions, the degree of hydrolysis (DH) of the hydrolysates was 12.6 % when obtained with Alcalase and approximately 4 % with Neutrase or bromelain. Post-hydrolysis polymerization did not result in an increase in molecular mass of the protein, but these samples presented a lower DH, determined by trinitrobenzenosulfonic acid (TNBS) method, suggesting that polymerization had occurred. Hydrolysis with the three enzymes reduced the beta-Lg antigenicity, as evaluated by ELISA and immunoblotting analyses. The IgE-binding responses were practically null (< 9 mu g mL(-1)), 22.82 and 55.73 mu g mL(-1) towards the hydrolysates obtained with Alcalase, bromelain, and Neutrase, respectively. The post-hydrolysis polymerization increased or had no significant effect (P a parts per thousand yen 0.05) on the antigenic response of the hydrolysates. (AU)

• Processo FAPESP: 09/51580-1 - Efeito da polimerizacao por transglutaminase e da proteolise na estrutura e antigenicidade da beta-lactoglobulina
• Beneficiário: Flavia Maria Netto
• Modalidade de apoio: Auxílio à Pesquisa - Regular