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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Photodynamic inactivation of biofilms formed by Candida spp., Trichosporon mucoides, and Kodamaea ohmeri by cationic nanoemulsion of zinc 2,9,16,23-tetrakis(phenylthio)-29H, 31H-phthalocyanine (ZnPc)

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Autor(es):
Junqueira, J. C. [1] ; Jorge, A. O. C. [1] ; Barbosa, J. O. [1] ; Rossoni, R. D. [1] ; Vilela, S. F. G. [1] ; Costa, A. C. B. P. [1] ; Primo, F. L. [2] ; Goncalves, J. M. [2] ; Tedesco, A. C. [2] ; Suleiman, J. M. A. H. [3]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] UNESP Univ Estadual Paulista, Dept Biosci & Oral Diag, Sch Dent Sao Jose dos Campos, BR-12245000 Sao Jose Dos Campos 12245000, SP - Brazil
[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Lab Fotobiol & Fotomed, Dept Quim, Ctr Nanotecnol & Engn Tecidual Ribeirao Preto, BR-14040901 Ribeirao Preto, SP - Brazil
[3] Emilio Ribas Inst Infectol, BR-01246900 Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Lasers in Medical Science; v. 27, n. 6, p. 1205-1212, NOV 2012.
Citações Web of Science: 32
Resumo

The biofilms formed by opportunistic yeasts serve as a persistent reservoir of infection and impair the treatment of fungal diseases. The aim of this study was to evaluate photodynamic inactivation (PDI) of biofilms formed by Candida spp. and the emerging pathogens Trichosporon mucoides and Kodamaea ohmeri by a cationic nanoemulsion of zinc 2,9,16,23-tetrakis(phenylthio)-29H,31H-phthalocyanine (ZnPc). Biofilms formed by yeasts after 48 h in the bottom of 96-well microtiter plates were treated with the photosensitizer (ZnPc) and a GaAlAs laser (26.3 J cm(-2)). The biofilm cells were scraped off the well wall, homogenized, and seeded onto Sabouraud dextrose agar plates that were then incubated at 37A degrees C for 48 h. Efficient PDI of biofilms was verified by counting colony-forming units (CFU/ml), and the data were submitted to analysis of variance and the Tukey test (p < 0.05). All biofilms studied were susceptible to PDI with statistically significant differences. The strains of Candida genus were more resistant to PDI than emerging pathogens T. mucoides and K. ohmeri. A mean reduction of 0.45 log was achieved for Candida spp. biofilms, and a reduction of 0.85 and 0.84, were achieved for biofilms formed by T. mucoides and K. ohmeri, respectively. Therefore, PDI by treatment with nanostructured formulations cationic zinc 2,9,16,23- tetrakis (phenylthio)- 29H, 31H- phthalocyanine (ZnPc) and a laser reduced the number of cells in the biofilms formed by strains of C. albicans and non-Candida albicans as well the emerging pathogens T. mucoides and K. ohmeri. (AU)

Processo FAPESP: 09/52283-0 - Diversidade genética e sensibilidade a fotossensibilização in vitro de Candida albicans isoladas de pacientes com candidose orofaríngea
Beneficiário:Juliana Campos Junqueira
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 09/15363-6 - Desenvolvimento e caracterização de equivalentes dérmicos modificados com células tronco mesenquimais como modelos para avaliação de matriz extracelular após fotoestímulo com diodo-laser de baixa potência
Beneficiário:Fernando Lucas Primo
Linha de fomento: Bolsas no Brasil - Pós-Doutorado