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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

DEVELOPMENT AND CHARACTERIZATION OF NINE MICROSATELLITE LOCI FOR SISYRINCHIUM MICRANTHUM (IRIDACEAE)

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Autor(es):
Tacuatia, Luana O. [1] ; Cidade, Fernanda W. [2] ; Souza, Anete P. [3, 2] ; Souza-Chies, Tatiana T. [4]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Fed Rio Grande do Sul, Inst Biociencias, Programa Posgrad Genet & Biol Mol, BR-91501970 Porto Alegre, RS - Brazil
[2] Univ Estadual Campinas, CBMEG, BR-13083970 Campinas, SP - Brazil
[3] Univ Estadual Campinas, Inst Biol, Dept Biol Vegetal, BR-13083970 Campinas, SP - Brazil
[4] Univ Fed Rio Grande do Sul, Inst Biociencias, Dept Bot, BR-91501970 Porto Alegre, RS - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: AMERICAN JOURNAL OF BOTANY; v. 99, n. 10, p. E402-E404, OCT 2012.
Citações Web of Science: 1
Resumo

Premise of the study: Sisyrinchium micranthum is an herb with wide distribution and morphological variation, presenting three known cytotypes (2x, 4x, and 6x), which is naturally occurring from Mexico to Argentina. The development of microsatellite markers for this species is important to provide molecular tools to investigate polyploidy origin, as well as its relationship with phenotypic and genetic variability. Methods and Results: Nine microsatellite markers were isolated using an enriched genomic library and characterized in a diploid population. Eight markers generated polymorphic loci in 27 plants of a population from southern Brazil. The number of alleles per locus ranged from two to nine, and the observed and expected heterozygosities varied from 0.192 to 0.741 and from 0.370 to 0.884, respectively. Conclusions: These species-specific DNA markers may be useful for further investigations of the involved polyploidization process and the population genetics of S. micranthum. (AU)

Processo FAPESP: 06/61242-8 - Desenvolvimento e caracterização de marcadores moleculares microssatélites em espécies forrageiras nativas
Beneficiário:Fernanda Witt Cidade
Linha de fomento: Bolsas no Brasil - Doutorado