The Fungal Pathogen Moniliophthora perniciosa Has Genes Similar to Plant PR-1 That Are Highly Expressed during Its Interaction with Cacao

Autor(es)

Teixeira, Paulo J. P. L. ^[1] ; Thomazella, Daniela P. T. ^[1] ; Vidal, Ramon O. ^{[1, 2]} ; do Prado, Paula F. V. ^[1] ; Reis, Osvaldo ^[1] ; Baroni, Renata M. ^{[1, 3]} ; Franco, Sulamita F. ^[1] ; Mieczkowski, Piotr ^[4] ; Pereira, Goncalo A. G. ^[1] ; Mondego, Jorge M. C. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Genet Evolucao & Bioagentes, Sao Paulo - Brazil

^[2] Lab Nacl Biociencias, Sao Paulo - Brazil

^[3] Inst Agron Campinas, Ctr Pesquisa & Desenvolvimento Recursos Genet Veg, Sao Paulo - Brazil

^[4] Univ N Carolina, Sch Med, Dept Genet, Chapel Hill, NC - USA

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 7, n. 9 SEP 20 2012.
• Citações Web of Science: 16

Resumo

The widespread SCP/TAPS superfamily (SCP/Tpx-1/Ag5/PR-1/Sc7) has multiple biological functions, including roles in the immune response of plants and animals, development of male reproductive tract in mammals, venom activity in insects and reptiles and host invasion by parasitic worms. Plant Pathogenesis Related 1 (PR-1) proteins belong to this superfamily and have been characterized as markers of induced defense against pathogens. This work presents the characterization of eleven genes homologous to plant PR-1 genes, designated as MpPR-1, which were identified in the genome of Moniliophthora perniciosa, a basidiomycete fungus responsible for causing the devastating witches' broom disease in cacao. We describe gene structure, protein alignment and modeling analyses of the MpPR-1 family. Additionally, the expression profiles of MpPR-1 genes were assessed by qPCR in different stages throughout the fungal life cycle. A specific expression pattern was verified for each member of the MpPR-1 family in the conditions analyzed. Interestingly, some of them were highly and specifically expressed during the interaction of the fungus with cacao, suggesting a role for the MpPR-1 proteins in the infective process of this pathogen. Hypothetical functions assigned to members of the MpPR-1 family include neutralization of plant defenses, antimicrobial activity to avoid competitors and fruiting body physiology. This study provides strong evidence on the importance of PR-1-like genes for fungal virulence on plants. (AU)

• Processo FAPESP: 07/50262-0 - Clonagem e analise de expressao de um gene pertencente a familia pr-1 expresso pelo fungo moniliophthora perniciosa causador da vassoura de bruxa do cacaueiro.
• Beneficiário: Paulo José Pereira Lima Teixeira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 09/51018-1 - Construção de um atlas transcriptômico para o estudo da doença vassoura-de-bruxa do cacaueiro
• Beneficiário: Paulo José Pereira Lima Teixeira
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 06/53553-3 - Caracterização de proteinases expressas pelo fungo causador da vassoura de bruxa, Crinipellis perniciosa
• Beneficiário: Jorge Maurício Costa Mondego
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 09/50119-9 - Estudo integrado e comparativo de três doenças fúngicas do cacau: vassoura-de-bruxa, monilíase e mal do facão, visando à compreensão de mecanismos de patogenicidade para o desenvolvimento de estratégias de controle
• Beneficiário: Gonçalo Amarante Guimarães Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Temático