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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Silibinin modulates the NF-kappa b pathway and pro-inflammatory cytokine production by mononuclear cells from preeclamptic women

Texto completo
Autor(es):
Innocenti Giorgi, Vanessa S. [1] ; Peracoli, Maria Terezinha S. [1] ; Peracoli, Jose C. [2] ; Witkin, Steven S. [2, 3] ; Bannwart-Castro, Camila F. [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ, Inst Biosci, Dept Microbiol & Immunol, Botucatu, SP - Brazil
[2] Sao Paulo State Univ, Dept Gynecol & Obstet, Botucatu, SP - Brazil
[3] Cornell Univ, Dept Obstet & Gynecol, Weill Med Coll, New York, NY 10021 - USA
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF REPRODUCTIVE IMMUNOLOGY; v. 95, n. 1-2, p. 67-72, SEP 2012.
Citações Web of Science: 34
Resumo

Preeclampsia (PE) is a complication of human pregnancy associated with an intense inflammatory response involving leukocyte activation, as well as elevated production of pro-inflammatory cytokines. The nuclear transcription factor-kappa B (NF-kappa B) is present in cells of the immune system and is responsible for transcription of genes coding for pro-inflammatory proteins. Silibinin is the main component of silymarin, a polyphenolic extract obtained from fruits and seeds of Silybum marianum with potent hepatoprotective and anti-inflammatory activities. In this study, we assessed whether silibinin modulated NF-kappa B activity and the production of pro-inflammatory cytokines by peripheral blood mononuclear cells (PBMCs) from preeclamptic patients. PBMC from women with PE, normotensive (NT) pregnant women, and nonpregnant (NP) women were cultured with or without silibinin (5 mu M and 50 mu M) and 1 mu g/mL lipopolysaccharide (LPS) for 18 h. The supernatants were assayed for tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) by ELISA. Cells were cultured for 30 min to evaluate NF-kappa B activity. There was increased endogenous activation of NF-kappa B as well as TNF-alpha and IL-1 beta release by PBMC in the PE group compared with the NT and NP groups. A positive correlation between NF-kappa B activity and cytokine production was also observed in the PE group. Silibinin was capable of reducing, at least in part, the levels of NF-kappa B and cytokines TNF-alpha and IL-1 beta in preeclamptic women. We conclude that silibinin exhibits potent anti-inflammatory activity on PBMC from preeclamptic women by downmodulation of NF-kappa B activation and inflammatory cytokine production. (C) 2012 Elsevier Ireland Ltd. All rights reserved. (AU)

Processo FAPESP: 10/00776-0 - Efeito da silibinina sobre o fator de transcrição nuclear-kB e produção de citocinas inflamatórias por células mononucleares de gestantes portadoras de pré-eclâmpsia
Beneficiário:Vanessa Silvestre Innocenti Giorgi
Linha de fomento: Bolsas no Brasil - Iniciação Científica