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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

TWO SEQUENTIAL PCR AMPLIFICATIONS FOR DETECTION OF Schistosoma mansoni IN STOOL SAMPLES WITH LOW PARASITE LOAD

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Autor(es):
Carvalho do Espirito-Santo, Maria Cristina [1] ; Alvarado-Mora, Monica Viviana [2] ; Silva Pinto, Pedro Luiz [3] ; Carrilho, Flair Jose ; Rebello Pinho, Joao Renato [2] ; Borges Gryschek, Ronaldo Cesar [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Med, Dept Infect & Parasit Dis, Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Med, Lab Gastroenterol & Hepatol, Inst Trop Med, Dept Gastroenterol, Sao Paulo - Brazil
[3] Adolfo Lutz Inst, Enteroparasitosis Sect, Parasitol & Mycol Div, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Revista do Instituto de Medicina Tropical de São Paulo; v. 54, n. 5, p. 245-248, SEP-OCT 2012.
Citações Web of Science: 7
Resumo

Schistosomiasis constitutes a major public health problem, with an estimated 200 million individuals infected worldwide and 700 million people living in risk areas. In Brazil there are areas of high, medium and low endemicity. Studies have shown that in endemic areas with a low prevalence of Schistosoma infection the sensitivity of parasitological methods is clearly reduced. Consequently diagnosis is often impeded due to the presence of false-negative results. The aim of this study is to present the PCR reamplification (Re-PCR) protocol for the detection of Schistosoma mansoni in samples with low parasite load (with less than 100 eggs per gram (epg) of feces). Three methods were used for the lysis of the envelopes of the S. mansoni eggs and two techniques of DNA extraction were carried out. Extracted DNA was quantified, and the results suggested that the extraction technique, which mixed glass beads with a guanidine isothiocyanate/phenol/chloroform (GT) solution, produced good results. PCR reamplification was conducted and detection sensitivity was found to be five eggs per 500 mg of artificially marked feces. The results achieved using these methods suggest that they are potentially viable for the detection of Schistosoma infection with low parasite load. (AU)

Processo FAPESP: 07/53457-7 - Estudo da variabilidade genotípica dos vírus das Hepatites B e C na Colômbia
Beneficiário:João Renato Rebello Pinho
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 08/50461-6 - Estudo da variabilidade genotípica dos vírus da Hepatite B e Hepatite C na Colômbia
Beneficiário:Monica Viviana Alvarado Mora
Linha de fomento: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 10/52615-0 - Estudo comparativo de métodos diagnósticos da esquistossomose mansoni em áreas de baixa transmissão e prevalência
Beneficiário:Ronaldo Cesar Borges Gryschek
Linha de fomento: Auxílio à Pesquisa - Regular