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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Effects of swim training on liver carcinogenesis in male Wistar rats fed a low-fat or high-fat diet

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Autor(es):
Aguiar e Silva, Marco Aurelio [1] ; Vechetti-Junior, Ivan Jose [1] ; do Nascimento, Andre Ferreira [2] ; Furtado, Kelly Silva [3] ; Azevedo, Luciana [4] ; Ribeiro, Daniel Araki [5] ; Barbisan, Luis Fernando [6]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ, UNESP, Inst Biosci, Postgrad Program Gen & Appl Biol, BR-18618970 Botucatu, SP - Brazil
[2] Sao Paulo State Univ, UNESP, Sch Med, Dept Clin Med, BR-18618970 Botucatu, SP - Brazil
[3] Sao Paulo State Univ, UNESP, Sch Med, Dept Pathol, BR-18618970 Botucatu, SP - Brazil
[4] Univ Fed Alfenas, UNIFAL, Fac Nutr, BR-37130000 Alfenas, MG - Brazil
[5] Univ Fed Sao Paulo, UNIFESP, Dept Biosci, BR-11060001 Santos, SP - Brazil
[6] Sao Paulo State Univ, UNESP, Inst Biosci, Dept Morphol, BR-18618970 Botucatu, SP - Brazil
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: APPLIED PHYSIOLOGY NUTRITION AND METABOLISM; v. 37, n. 6, p. 1101-1109, DEC 2012.
Citações Web of Science: 6
Resumo

The present study aimed to investigate the beneficial effects of swim training on the promotion-progression stages of rat liver carcinogenesis. Male Wistar rats were submitted to chemically induced liver carcinogenesis and allocated into 4 major groups, according their dietary regimen (16 weeks) and swim training of 5 days per week (8 weeks): 2 groups were fed low-fat diet (LFD, 6% fat) and trained or not trained and 2 groups were fed high-fat diet (HFD, 21% fat) and trained or not trained. At week 20, the animals were killed and liver samples were processed for histological analyses; immunohistochemical detection of persistent or remodeling preneoplastic lesions (pPNL and rPNL) expressing placental glutathione S-transferase (GST-P) enzyme; or proliferating cell nuclear antigen (PCNA), cleaved caspase-3, and bcl-2 protein levels by Western blotting or malonaldehyde (MDA) and total glutathione detection by HPLC. Overall analysis indicated that swim training reduced the body weight and body fat in both LFD and HFD groups, normalized total cholesterol levels in the HFD group while decreased the MDA levels, increased glutathione levels and both number of GST-P-positive pPNL and hepatocellular adenomas in LFD group. Also, a favorable balance in PCNA, cleaved caspase-3, and bcl-2 levels was detected in the liver from the LFD-trained group in relation to LFD-untrained group. The findings of this study indicate that the swim training protocol as a result of exercise postconditioning may attenuate liver carcinogenesis under an adequate dietary regimen with lowered fat intake. (AU)

Processo FAPESP: 10/03056-9 - Efeitos do treinamento aeróbio na etapa de promoção da hepatocarcinogênese química em ratos Wistar obesos
Beneficiário:Marco Aurélio de Aguiar e Silva
Linha de fomento: Bolsas no Brasil - Mestrado