Enhanced Architecture of Lipid-Carbon Nanotubes as Langmuir-Blodgett Films to Investigate the Enzyme Activity of Phospholipases from Snake Venom

Autor(es)

Caseli, Luciano ^[1] ; Tiburcio, Vera L. B. ^[2] ; Vargas, Frey F. R. ^[3] ; Marangoni, Sergio ^[3] ; Siqueira, Jr., Jose R. ^[2]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Paulo UNIFESP, BR-0997227 Sao Paulo - Brazil

^[2] Fed Univ Triangulo Mineiro UFTM, BR-38025180 Uberaba, MG - Brazil

^[3] State Univ Campinas UNICAMP, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Physical Chemistry B; v. 116, n. 45, p. 13424-13429, NOV 15 2012.
• Citações Web of Science: 6

Resumo

The immobilization of biomolecules in films with a controlled architecture permits the access of information on the molecular interactions, not only between film components, but also between the film and substances in the external environment. In this study, we investigated the immobilization of the phospholipase A(2) from snake venoms (4-nitro-3-(octanoyloxy)benzoic acid, OBZ) in solid supports as a Langmuir-Blodgett (LB) film, followed by incorporation of carbon nanotubes (CNTs). The hybrid film was characterized by infrared spectroscopy and the interactions with its catalytic substrate were investigated The presence of CNTs leads to a structure with an adequate environment to preserve the enzyme properties, leading to an optimum catalytic activity. This enhanced architecture was exploited in terms of vibrational spectroscopy, which indicated changes in the secondary structure of the enzyme upon contact with the catalytic substrate. (AU)

• Processo FAPESP: 08/10851-0 - Filmes ultrafinos para a fabricação de biossensores e mimetização de biomembranas: aplicações na área ambiental e de saúde
• Beneficiário: Luciano Caseli
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores