Living between two worlds: two-phase culture systems for producing plant secondary metabolites

Autor(es)

Malik, Sonia ^[1] ; Mirjalili, Mohammad Hossein ^[2] ; Fett-Neto, Arthur Germano ^[3] ; Mazzafera, Paulo ^[1] ; Bonfill, Mercedes ^[4]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Biol, Dept Plant Biol, Campinas, SP - Brazil

^[2] Shahid Beheshti Univ, Dept Agr, Med Plants & Drugs Res Inst, Tehran - Iran

^[3] Ctr Biotechnol UFRGS, Plant Physiol Lab, Porto Alegre, RS - Brazil

^[4] Univ Barcelona, Fac Pharm, Plant Physiol Lab, E-08028 Barcelona - Spain

• Tipo de documento: Artigo de Revisão
• Fonte: CRITICAL REVIEWS IN BIOTECHNOLOGY; v. 33, n. 1, p. 1-22, MAR 2013.
• Citações Web of Science: 32

Resumo

The two-phase culture system is an important in vitro strategy to increase the production of secondary metabolites (SMs) by providing an enhanced release of these compounds from plant cells. Whereas the first phase supports cell growth, the second phase provides an additional site or acts as a metabolic sink for the accumulation of SMs and also reduces feedback inhibition. This review is focused on several aspects of the two-phase culture system and aims to show the diverse possibilities of employing this technique for the in vitro production of SMs from plant cells. Depending on the material used in the secondary phase, two-phase culture systems can be broadly categorised as liquid-liquid or liquid-solid. The choice of material for the second phase depends on the type of compound to be recovered and the compatibility with the other phase. Different factors affecting the efficiency of two-phase culture systems include the choice of material for the secondary phase, its concentration, volume, and time of addition. Factors such as cell elicitation, immobilization, and permeabilization, have been suggested as important strategies to make the two-phase culture system practically reliable on a commercial scale. Since there are many possibilities for designing a two-phase system, more detailed studies are needed to broaden the range of secondary phases compatible with the various plant species producing SMs with potential applications, mainly in the food and pharmacology industries. (AU)

• Processo FAPESP: 09/53450-8 - Studies on effect of root temperature on alkaloid production in Catharanthus roseus, Coffea arabica and Nicotiana tabacum
• Beneficiário: Sonia Malik
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado