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Galleria mellonella as a model host to study Paracoccidioides lutzii and Histoplasma capsulatum

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Thomaz, Luciana [1] ; Garcia-Rodas, Rocio [2] ; Guimaraes, Allan J. [3, 4] ; Taborda, Carlos P. [1] ; Zaragoza, Oscar [2] ; Nosanchuk, Joshua D. [3, 4]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil
[2] Inst Salud Carlos III, Natl Ctr Microbiol, Mycol Reference Lab, Madrid - Spain
[3] Albert Einstein Coll Med, Dept Microbiol & Immunol, Bronx, NY 10467 - USA
[4] Albert Einstein Coll Med, Dept Med, Bronx, NY 10467 - USA
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: VIRULENCE; v. 4, n. 2, p. 139-146, FEB 15 2013.
Citações Web of Science: 41

Non-mammalian models have been used to investigate fungal virulence. In this work we have explored the use of Galleria mellonella as an infection model for the pathogenic dimorphic fungi Histoplasma capsulatum and Paracoccidioides lutzii. In mammalian models these fungi cause similar infections, and disease outcomes are influenced by the quantity of the infective inocula. We describe a similar aspect in a G. mellonella model and characterize the pathogenesis features in this system. Infection with P. lutzii or H. capsulatum, in all inoculum used, killed larvae at 25 and 37 degrees C. However, there was a lack of correlation between the number of yeast cells used for infection and the time to larvae death, which may indicate that the fungi induce protective responses in a dynamic manner as the lowest concentrations of fungi induced the most rapid death. For both fungi, the degree of larvae melanization was directly proportional to the inocula size, and this effect was visibly more apparent at 37 degrees C. Histological evaluation of the larvae showed a correlation between the inoculum and granuloma-like formation. Our results suggest that G. mellonella is a potentially useful model to study virulence of dimorphic fungi. (AU)

Processo FAPESP: 07/53175-1 - Eficácia e função biológica de anticorpos monoclonais gerados contra antígenos de parede e exoantígenos de Paracoccidoides brasiliensis na infecção experimental
Beneficiário:Luciana Thomaz
Linha de fomento: Bolsas no Brasil - Doutorado