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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Phylogenetic Analysis and DNA-based Species Confirmation in Anopheles (Nyssorhynchus)

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Autor(es):
Foster, Peter G. [1] ; Bergo, Eduardo S. [2] ; Bourke, Brian P. [3] ; Oliveira, Tatiane M. P. [3] ; Nagaki, Sandra S. [3] ; Sant'Ana, Denise C. [3] ; Sallum, Maria Anice M. [3]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Nat Hist Museum, Dept Life Sci, London SW7 5BD - England
[2] Secretaria Estado Saude Sao Paulo, Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Saude Publ, Dept Epidemiol, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 8, n. 2 FEB 4 2013.
Citações Web of Science: 44
Resumo

Specimens of neotropical Anopheles (Nyssorhynchus) were collected and identified morphologically. We amplified three genes for phylogenetic analysis-the single copy nuclear white and CAD genes, and the COI barcode region. Since we had multiple specimens for most species we were able to test how well the single or combined genes were able to corroborate morphologically defined species by placing the species into exclusive groups. We found that single genes, including the COI barcode region, were poor at confirming species, but that the three genes combined were able to do so much better. This has implications for species identification, species delimitation, and species discovery, and we caution that single genes are not enough. Higher level groupings were partially resolved with some well-supported groupings, whereas others were found to be either polyphyletic or paraphyletic. There were examples of known groups, such as the Myzorhynchella Section, which were poorly supported with single genes but were well supported with combined genes. From this we can infer that more sequence data will be needed in order to show more higher-level groupings with good support. We got unambiguously good support (0.94-1.0 Bayesian posterior probability) from all DNA-based analyses for a grouping of An. dunhami with An. nuneztovari and An. goeldii, and because of this and because of morphological similarities we propose that An. dunhami be included in the Nuneztovari Complex. We obtained phylogenetic corroboration for new species which had been recognised by morphological differences; these will need to be formally described and named. (AU)

Processo FAPESP: 11/20397-7 - Taxonomia morfológica e molecular e filogenia do subgênero Nyssorhynchus do gênero Anopheles (Diptera: Culicidae), com ênfase especial em Anopheles darlingi da Mata Atlântica
Beneficiário:Maria Anice Mureb Sallum
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 05/53973-0 - Sistemática de Anopheles (Nyssorhynchus) (Diptera: Culicidae)
Beneficiário:Maria Anice Mureb Sallum
Linha de fomento: Auxílio à Pesquisa - Temático