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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Ultrastructural and morphometric alterations in the aortic depressor nerve of rats due to long term experimental diabetes: Effects of insulin treatment

Texto completo
Autor(es):
Oliveira, Fabricio S. [1] ; Nessler, Randy A. [2] ; Castania, Jaci A. [3] ; Salgado, Helio C. [3] ; Fazan, Valeria P. S. [2, 3]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Estadual Maringa, Umuarama, Parana - Brazil
[2] Univ Iowa, Cent Microscopy Res Facil, Iowa City, IA - USA
[3] Univ Sao Paulo, Sch Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Brain Research; v. 1491, p. 197-203, JAN 23 2013.
Citações Web of Science: 11
Resumo

Most of the reports about an altered baroreflex attribute this condition to the diabetic efferent neuropathy of the aortic depressor nerve (ADN) (afferent arm of the baroreflex less explored). We evaluated the ADN ultrastructural alterations caused by long term experimental diabetes and the effects of insulin treatment. Wistar rats (N=14) received a single intravenous injection of streptozotocin (40 mg/kg) 12 weeks before the experiment. Control animals (N=9) received vehicle (citrate buffer). Insulin treated animals (N=8) received a single subcutaneous injection of insulin daily. Under pentobarbital anesthesia the ADNs were isolated and had their spontaneous activity recorded. Afterwards, proximal and distal segments of the nerves were prepared for transmission electron microscopy study. Morphometry of the unmyelinated fibers was carried out with the aid of computer software. ADN of the diabetic animals showed axonal atrophy for myelinated fibers, with more pronounced alterations of the myelin sheath, such as myelin infolding and out folding, presence of myelin balls and very thin myelin sheath in relation to the axonal size, particularly for the small myelinated fibers becoming evident. No differences were observed in myelinated fiber number and their density, as well as on the fascicular area. Unmyelinated fiber number was significantly larger in the diabetic group while fiber diameter was significantly smaller compared to control. This result suggests axonal atrophy or, if associated to the larger number of fibers present in this group, could indicate fiber sprouting. These alterations were more evident in the distal segments of the nerves and were moderated by insulin treatment. (C) 2012 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 09/50389-6 - Regulação cardiocirculatória em condições fisiológicas e fisiopatológicas
Beneficiário:Helio Cesar Salgado
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 09/16748-9 - Avaliação morfológica e morfométrica do nervo frênico de ratos espontaneamente hipertensos em diferentes fases do desenvolvimento
Beneficiário:Valéria Paula Sassoli Fazan
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 10/12518-6 - Avaliação morfológica e comparação dos tipos de fibras dos músculos gastrocnêmios lateral e medial e flexor longo do hálux após estimulação dolorosa no período neonatal
Beneficiário:Valéria Paula Sassoli Fazan
Linha de fomento: Auxílio à Pesquisa - Regular