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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Experimental infection of suckling mice by subcutaneous inoculation with Oropouche virus

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Autor(es):
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Santos, Rodrigo I. [1] ; Almeida, Mariana F. P. [1] ; Paula, Flavia E. [1] ; Rodrigues, Alcir Humberto [1] ; Saranzo, Ariane Mattioli [1] ; Paula, Andre E. [1] ; Silva, Maria Lucia [1] ; Alves Correa, Vani Maria [1] ; Acrani, Gustavo Olszanski [1] ; Neder, Luciano [2] ; Arruda, Eurico [1]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Cell Biol, Sch Med, BR-14049 Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Dept Pathol, Sch Med, BR-14049 Ribeirao Preto - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: VIRUS RESEARCH; v. 170, n. 1-2, p. 25-33, DEC 2012.
Citações Web of Science: 11
Resumo

Oropouche virus, of the family Bunyaviridae, genus Orthobunyavirus, serogroup Simbu, is an important causative agent of arboviral febrile illness in Brazil. An estimated 500,000 cases of Oropouche fever have occurred in Brazil in the last 30 years, with recorded cases also in Panama, Peru, Suriname and Trinidad. We have developed an experimental model of Oropouche virus infection in neonatal BALB/c mouse by subcutaneous inoculation. The vast majority of infected animals developed disease on the 5th day post infection, characterized mainly by lethargy and paralysis, progressing to death within 10 days. Viral replication was documented in brain cells by in situ hybridization, immunohistochemistry and virus titration. Multi-step immunohistochemistry indicated neurons as the main target cells of OROV infection. Histopathology revealed glial reaction and astrocyte activation in the brain and spinal cord, with neuronal apoptosis. Spleen hyperplasia and mild meningitis were also found, without viable virus detected in liver and spleen. This is the first report of an experimental mouse model of OROV infection, with severe involvement of the central nervous system, and should become useful in pathogenesis studies, as well as in preclinical testing of therapeutic interventions for this emerging pathogen. (c) 2012 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 08/50617-6 - Estudos sobre vírus emergentes incluindo arbovirus, robovirus, vírus respiratórios e de transmissão congênita, no Centro de Pesquisa em Virologia da Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo
Beneficiário:Luiz Tadeu Moraes Figueiredo
Linha de fomento: Auxílio à Pesquisa - Temático