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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

ENDOGLUCANASE PRODUCTION WITH THE NEWLY ISOLATED Myceliophtora sp I-1D3b IN A PACKED BED SOLID STATE FERMENTOR

Texto completo
Autor(es):
Zanelato, A. I. [1] ; Shiota, V. M. [1] ; Gomes, E. [2] ; da Silva, R. [2] ; Thomeo, J. C. [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Dept Engn & Tecnol Alimentos, Sao Jose Do Rio Preto, SP - Brazil
[2] Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Lab Bioquim & Microbiol Aplicada, Sao Jose Do Rio Preto, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Brazilian Journal of Microbiology; v. 43, n. 4, p. 1536-1544, OCT-DEC 2012.
Citações Web of Science: 13
Resumo

This work is aimed to produce endoglucanase through solid state fermentation in a packed bed bioreactor with the use of the fungus Myceliophtora sp. I-1D3b using a mixture of wheat bran (WB) and sugar cane bagasse (SCB) as culture medium. Preliminary tests were performed in polypropylene plastic bags, controlling the variables temperature (40, 45, and 50 degrees C), initial moisture content (75, 80, and 85%, w.b.), and weight proportion SCB/WB (1: 1, 7: 3, and 9: 1). The highest enzyme activities in plastic bags were obtained using the substrate proportion of 7: 3, 50 degrees C temperature, and 80% initial moisture content (878 U/grams of dry solid). High activities of filter-paper cellulase and xylanase were also obtained in plastic bags and some results are reported. For the packed bed experiments, the temperature (45 and 50 degrees C) and the air flow rate (80, 100 and 120L/h) were the controlled variables. Activity of endoglucanase was similar to plastic bag tests. A longitudinal gradient of moisture content, was observed increasing from the bottom to the top of the reactor, even though the longitudinal enzyme activity profile was flat for almost the whole bed. Air flow rate did not affect enzyme activity, while experiments carried out at 50 degrees C showed higher enzyme activities. The maximum temperature peak observed was at about 6 degrees C above the process temperature. (AU)

Processo FAPESP: 10/12624-0 - Aplicação de métodos físico-químicos e enzimáticos na sacarificação do bagaço de cana: estudos de microrganismos, processos fermentativos relacionados e métodos de hidrólise
Beneficiário:Eleni Gomes
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 08/52811-4 - Produção de enzimas celulolíticas por fermentação em estado sólido em biorretor de leito fixo
Beneficiário:Alex Izuka Zanelato
Linha de fomento: Bolsas no Brasil - Mestrado