Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Influence of artificial saliva in biofilm formation of Candida albicans in vitro

Texto completo
Autor(es):
Silva, Michelle Peneluppi [1] ; Chibebe Junior, Jose [1] ; Jorjao, Adeline Lacerda [1] ; da Silva Machado, Ana Karina [1] ; de Oliveira, Luciane Dias [1] ; Junqueira, Juliana Campos [1] ; Cardoso Jorge, Antonio Olavo [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] UNESP Univ Estadual Paulista, Sch Dent Sao Jose dos Campos, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Brazilian Oral Research; v. 26, n. 1, p. 24-28, JAN-FEB 2012.
Citações Web of Science: 8
Resumo

Due to the increase in life expectancy, new treatments have emerged which, although palliative, provide individuals with a better quality of life. Artificial saliva is a solution that contains substances that moisten a dry mouth, thus mimicking the role of saliva in lubricating the oral cavity and controlling the existing normal oral microbiota. This study aimed to assess the influence of commercially available artificial saliva on biofilm formation by Candida albicans. Artificial saliva I consists of carboxymethylcellulose, while artificial saliva II is composed of glucose oxidase, lactoferrin, lysozyme and lactoperoxidase. A control group used sterile distilled water. Microorganisms from the oral cavity were transferred to Sabouraud Dextrose Agar and incubated at 37 degrees C for 24 hours. Colonies of Candida albicans were suspended in a sterile solution of NaCl 0.9%, and standardisation of the suspension to 10(6) cells/mL was achieved. The acrylic discs, immersed in artificial saliva and sterile distilled water, were placed in a 24-well plate containing 2 mL of Sabouraud Dextrose Broth plus 5% sucrose and 0.1 mL aliquot of the Candida albicans suspension. The plates were incubated at 37 degrees C for 5 days, the discs were washed in 2 mL of 0.9% NaCl and placed into a tube containing 10 mL of 0.9% NaCl. After decimal dilutions, aliquots of 0.1 mL were seeded on Sabouraud Dextrose Agar and incubated at 37 degrees C for 48 hours. Counts were reported as CFU/mL (Log10). A statistically significant reduction of 29.89% (1.45 CFU/mL) of Candida albicans was observed in saliva I when compared to saliva II (p = 0.002, considering p <= 0.05). (AU)

Processo FAPESP: 10/14434-4 - Terapia fotodinâmica em Esporos de Bacillus atrophaeus e Bacillus subtilis: estudos com laser, LED, azul de metileno, Rosa Bengala e verde malaquita
Beneficiário:Michelle Peneluppi Silva
Linha de fomento: Bolsas no Brasil - Mestrado