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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

A multi-locus approach to barcoding in the Anopheles strodei subgroup (Diptera: Culicidae)

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Autor(es):
Bourke, Brian Patrick [1] ; Oliveira, Tatiane Porangaba [1] ; Suesdek, Lincoln [2] ; Bergo, Eduardo Sterlino [3] ; Mureb Sallum, Maria Anice [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Saude Publ, Dept Epidemiol, BR-01255 Sao Paulo - Brazil
[2] Inst Butantan, Parasitol Lab, Sao Paulo - Brazil
[3] Secretaria Estado Saude Sao Paulo, Superintendencia Controle Endemias, Araraquara, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: PARASITES & VECTORS; v. 6, APR 19 2013.
Citações Web of Science: 30
Resumo

Background: The ability to successfully identify and incriminate pathogen vectors is fundamental to effective pathogen control and management. This task is confounded by the existence of cryptic species complexes. Molecular markers can offer a highly effective means of species identification in such complexes and are routinely employed in the study of medical entomology. Here we evaluate a multi-locus system for the identification of potential malaria vectors in the Anopheles strodei subgroup. Methods: Larvae, pupae and adult mosquitoes(n = 61) from the An. strodei subgroup were collected from 21 localities in nine Brazilian states and sequenced for the COI, ITS2 and white gene. A Bayesian phylogenetic approach was used to describe the relationships in the Strodei Subgroup and the utility of COI and ITS2 barcodes was assessed using the neighbor joining tree and ``best close match{''} approaches. Results: Bayesian phylogenetic analysis of the COI, ITS2 and white gene found support for seven clades in the An. strodei subgroup. The COI and ITS2 barcodes were individually unsuccessful at resolving and identifying some species in the Subgroup. The COI barcode failed to resolve An. albertoi and An. strodei but successfully identified approximately 92% of all species queries, while the ITS2 barcode failed to resolve An. arthuri and successfully identified approximately 60% of all species queries. A multi-locus COI-ITS2 barcode, however, resolved all species in a neighbor joining tree and successfully identified all species queries using the ``best close match{''} approach. Conclusions: Our study corroborates the existence of An. albertoi, An. CP Form and An. strodei in the An. strodei subgroup and identifies four species under An. arthuri informally named A-D herein. The use of a multi-locus barcode is proposed for species identification, which has potentially important utility for vector incrimination. Individuals previously found naturally infected with Plasmodium vivax in the southern Amazon basin and reported as An. strodei are likely to have been from An. arthuri C identified in this study. (AU)

Processo FAPESP: 11/20397-7 - Taxonomia morfológica e molecular e filogenia do subgênero Nyssorhynchus do gênero Anopheles (Diptera: Culicidae), com ênfase especial em Anopheles darlingi da Mata Atlântica
Beneficiário:Maria Anice Mureb Sallum
Linha de fomento: Auxílio à Pesquisa - Regular