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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Oleic, Linoleic and Linolenic Acids Increase ROS Production by Fibroblasts via NADPH Oxidase Activation

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Autor(es):
Hatanaka, Elaine [1, 2] ; Dermargos, Alexandre [3, 4] ; Hirata, Aparecida Emiko [5] ; Ramirez Vinolo, Marco Aurelio [1, 6] ; Carpinelli, Angelo Rafael [1] ; Newsholme, Philip [7, 8] ; Armelin, Hugo Aguirre [3, 4] ; Curi, Rui [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo - Brazil
[2] Cruzeiro do Sul Univ, Inst Phys Act & Sport Sci, Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Quim, Sao Paulo - Brazil
[4] Inst Butantan, Lab Especial Ciclo Celular, Sao Paulo - Brazil
[5] Univ Fed Sao Paulo, Dept Fisiol, Sao Paulo - Brazil
[6] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut & Bioagents, Sao Paulo - Brazil
[7] Univ Coll Dublin, Conway Inst, Sch Biomol & Biomed Sci, Dublin 2 - Ireland
[8] Curtin Univ, Sch Biomed Sci, Perth, WA - Australia
Número total de Afiliações: 8
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 8, n. 4 APR 8 2013.
Citações Web of Science: 20
Resumo

The effect of oleic, linoleic and gamma-linolenic acids on ROS production by 3T3 Swiss and Rat 1 fibroblasts was investigated. Using lucigenin-amplified chemiluminescence, a dose-dependent increase in extracellular superoxide levels was observed during the treatment of fibroblasts with oleic, linoleic and gamma-linolenic acids. ROS production was dependent on the addition of beta-NADH or NADPH to the medium. Diphenyleneiodonium inhibited the effect of oleic, linoleic and gamma-linolenic acids on fibroblast superoxide release by 79%, 92% and 82%, respectively. Increased levels of p47(phox) phosphorylation due to fatty acid treatment were detected by Western blotting analyses of fibroblast proteins. Increased p47(phox) mRNA expression was observed using real-time PCR. The rank order for the fatty acid stimulation of the fibroblast oxidative burst was as follows: gamma-linolenic > linoleic > oleic. In conclusion, oleic, linoleic and gamma-linolenic acids stimulated ROS production via activation of the NADPH oxidase enzyme complex in fibroblasts. (AU)

Processo FAPESP: 04/12466-5 - Função dos ácidos oléico, linoléico e a-linolênico nos mecanismos envolvidos no processo de cicatrização
Beneficiário:Elaine Hatanaka Dermargos
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 08/51273-9 - Dualidade funcional do FGF2 (Fibroblast Growth Factor 2) no controle do ciclo celular: mitógeno e supressor de tumor
Beneficiário:Hugo Aguirre Armelin
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 06/52043-1 - Função dos ácidos oleico,linoleico e gama-linoleico nos mecanismos envolvidos no processod e cicatrização
Beneficiário:Rui Curi
Linha de fomento: Auxílio à Pesquisa - Regular