Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Bioactive Fraction of Geopropolis from Melipona scutellaris Decreases Neutrophils Migration in the Inflammatory Process: Involvement of Nitric Oxide Pathway

Texto completo
Autor(es):
Franchin, Marcelo [1] ; da Cunha, Marcos Guilherme [1] ; Denny, Carina [1] ; Napimoga, Marcelo Henrique [2] ; Cunha, Thiago Mattar [3] ; Bueno-Silva, Bruno [1] ; de Alencar, Severino Matias [4] ; Ikegaki, Masaharu [5] ; Rosalen, Pedro Luiz [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Sch Dent Piracicaba, Dept Physiol Sci, BR-13414903 Piracicaba, SP - Brazil
[2] Sao Leopoldo Mandic Inst & Res Ctr, Lab Immunol & Mol Biol, BR-13045755 Campinas, SP - Brazil
[3] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Pharmacol, BR-14049900 Ribeirao Preto, SP - Brazil
[4] Univ Sao Paulo, Dept Agrifood Ind Food & Nutr, Luiz de Queiroz Coll Agr, BR-13418900 Piracicaba, SP - Brazil
[5] Univ Fed Alfenas, Sch Pharmaceut Sci, BR-37130000 Alfenas, MG - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Evidence-based Complementary and Alternative Medicine; 2013.
Citações Web of Science: 11
Resumo

The aim of this study was to evaluate the activity of the ethanolic extract of geopropolis (EEGP) from Melipona scutellaris and its fractions on the modulation of neutrophil migration in the inflammatory process, and the participation of nitric oxide (NO) pathway, as well as to check the chemical profile of the bioactive fraction. EEGP and its aqueous fraction decreased neutrophil migration in the peritoneal cavity and also the interaction of leukocytes (rolling and adhesion) with endothelial cells. The levels of chemokines CXCL1/KC and CXCL2/MIP-2 were not altered after treatment with EEGP and the aqueous fraction. It was found that the injection of NO pathway antagonists abolished the EEGP and the aqueous fraction inhibitory activity on the neutrophil migration. The expression of intercellular adhesion molecule type 1 (ICAM-1) was reduced, and nitrite levels increased after treatment with EEGP and aqueous fraction. In the carrageenan-induced paw edema model, EEGP and the aqueous fraction showed antiedema activity. No pattern of flavonoid and phenolic acid commonly found in propolis samples of Apis mellifera could be detected in the aqueous fraction samples. These data indicate that the aqueous fraction found has promising bioactive substances with anti-inflammatory activity. (AU)

Processo FAPESP: 09/12352-3 - Avaliação do potencial anti-inflamatório e analgésico da própolis de meliponinea (Melipona scutellaris)
Beneficiário:Marcelo Franchin
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 10/20214-7 - Identificação e caracterização química da fração bioativa e nanoestruturada da geoprópolis de Meliponinea (Melipona scutellaris) e suas atividades farmacológicas relacionadas
Beneficiário:Pedro Luiz Rosalen
Linha de fomento: Auxílio à Pesquisa - Regular