The Inhibition of Inflammasome by Brazilian Propolis (EPP-AF)

Autor(es)

Hori, Juliana I. ^[1] ; Zamboni, Dario S. ^[1] ; Carrao, Daniel B. ^[2] ; Goldman, Gustavo Henrique ^{[3, 4]} ; Berretta, Andresa A. ^{[3, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, FMRP, Dept Biol Celular Mol & Bioagentes Patogen, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Apis Flora Ind & Comercial Ltda, BR-14020670 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Dept Ciencias Farmaceut, Fac Ciencias Farmaceut Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil

^[4] Lab Nacl Ciencia & Tecnol Bioetanol CTBE, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Evidence-based Complementary and Alternative Medicine; 2013.
• Citações Web of Science: 8

Resumo

Propolis extracts have gained the attention of consumers and researchers due to their unique chemical compositions and functional properties such as its anti-inflammatory activity. Recently, it was described a complex that is also important in inflammatory processes, named inflammasome. The inflammasomes are a large molecular platform formed in the cell cytosol in response to stress signals, toxins, and microbial infections. Once activated, the inflammasome induces caspase-1, which in turn induces the processing of inflammatory cytokines such as IL-1 beta. and IL-18. So, to understand inflammasomes regulation becomes crucial to treat several disorders including autoinflammatory diseases. Since green propolis extracts are able to regulate inflammatory pathways, this work purpose was to investigate if this extract could also act on inflammasomes regulation. First, the extract was characterized and it demonstrated the presence of important compounds, especially Artepillin C. This extract was effective in reducing the IL-1 beta. secretion in mouse macrophages and this reduction was correlated with a decrease in activation of the protease caspase-1. Furthermore, we found that the extract at a concentration of 30 mu g/mL was not toxic to the cells even after a 18-hour treatment. Altogether, these data indicate that Brazilian green propolis (EPP-AF) extract has a role in regulating the inflammasomes. (AU)

• Processo FAPESP: 11/51023-5 - O papel de transportadores de potássio bacterianos no processo de ativação do inflamassoma e na resposta de macrófagos a infecção por Legionella pneumophila
• Beneficiário: Juliana Issa Hori
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 06/52867-4 - Reconhecimento de patógenos bacterianos por receptores intracelulares e sua importância no controle da infecção microbiana
• Beneficiário: Dario Simões Zamboni
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
• Processo FAPESP: 11/50089-2 - Obtenção de um insumo farmacêutico de origem natural para tratamento de candidemias e candidíases
• Beneficiário: Andresa Aparecida Berretta e Silva
• Linha de fomento: Auxílio à Pesquisa - Pesquisa Inovativa em Pequenas Empresas - PIPE