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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Enzymatic de-glycosylation of rutin improves its antioxidant and antiproliferative activities

Texto completo
Autor(es):
Melo Branco de Araujo, Maria Elisa [1] ; Moreira Franco, Yollanda E. [1] ; Alberto, Thiago Grando [1] ; Sobreiro, Mariana Alves [1] ; Conrado, Marco Aurelio [1] ; Priolli, Denise Goncalves [1] ; Frankland Sawaya, Alexandra C. H. [2] ; Ruiz, Ana Lucia T. G. [3] ; de Carvalho, Joao Ernesto [3] ; Carvalho, Patricia de Oliveira [1]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] USF, Lab Multidisciplinary Res, BR-12916900 Braganca Paulista, SP - Brazil
[2] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Plant Biol, BR-13083970 Campinas, SP - Brazil
[3] Univ Estadual Campinas, Ctr Pluridisciplinar Pesquisas Quim Biol & Agr, BR-13083970 Paulinia, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Food Chemistry; v. 141, n. 1, p. 266-273, NOV 1 2013.
Citações Web of Science: 34
Resumo

Bioavailability and biological properties of flavonoid glycosides can be improved after the enzymatic hydrolysis of specific glycosyl groups. In this study, we evaluate the antioxidant and antiproliferative potential of rutin after enzymatic hydrolysis performed by alpha-L-rhamnosidases (hesperidinase from Penicillium sp. and naringinase from Penicillium decumbens) previously heated at 70 degrees C for 30 min to inactivate the undesirable beta-D-glucosidase activity. The highest in vitro antioxidant activity determined by DPPH radical scavenging was achieved with rutin hydrolyzed by hesperidinase. Rutin was predominantly bioconverted into quercetin-3-glucoside. There was no statistical difference between xanthine oxidase inhibition by rutin before and after hydrolysis. However, in vitro inhibitory activity against ten human tumor cell lines showed that hydrolyzed rutin exerted a more potent antiproliferative effect than quercetin and rutin on various cancer cell lines, specially glioma, and ovarian and breast adenocarcinomas. These results indicate that quercetin-3-glucoside could be a promising functional derivative obtained by rutin hydrolysis. (c) 2013 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 09/09224-3 - Biotransformação de polifenóis e potencial antioxidante
Beneficiário:Patrícia de Oliveira Carvalho
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 11/12394-8 - Avaliação in vitro da atividade antioxidante e antimutagênica de flavonóides glicosilados e de suas agliconas
Beneficiário:Mariana Alves Sobreiro
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 08/58035-6 - Control of lignin biosynthesis in sugar cane: many gaps still to be filled
Beneficiário:Paulo Mazzafera
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático