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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

A flagellated motile Salmonella Gallinarum mutant (SG Fla(+)) elicits a pro-inflammatory response from avian epithelial cells and macrophages and is less virulent to chickens

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Autor(es):
de Freitas Neto, Oliveiro Caetano [1, 2] ; Setta, Ahmed [1, 3] ; Imre, Ariel [1, 4] ; Bukovinski, Agnes [1] ; Elazomi, Altaeb [1, 5] ; Kaiser, Pete [6] ; Berchieri Junior, Angelo [2] ; Barrow, Paul [1] ; Jones, Michael [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Nottingham, Sch Vet Med & Sci, Loughborough LE12 5RD, Leics - England
[2] Univ Sao Paulo State, Dept Vet Pathol, BR-14884900 Jaboticabal - Brazil
[3] Cairo Univ, Fac Vet Med, Dept Poultry Dis, Giza - Egypt
[4] CEVA Phylaxia Biotechnol Europe, H-1107 Budapest - Hungary
[5] Fac Med Technol, Med Labs Dept, Misurata - Libya
[6] Univ Edinburgh, Easter Bush Vet Ctr, Roslin Inst, Roslin EH25 9RG, Midlothian - Scotland
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: Veterinary Microbiology; v. 165, n. 3-4, p. 425-433, AUG 30 2013.
Citações Web of Science: 19
Resumo

Salmonella enterica subspecies enterica serovar Gallinarum biovar Gallinarum (SG) is a non-flagellated bacterium which causes fowl typhoid, a systemic disease associated with high mortality in birds. It has been suggested that the absence of flagella in SG is advantageous in the early stages of systemic infection through absence of TLR-5 activation. In order to investigate this hypothesis in more detail a flagellated and motile SG mutant (SG Fla(+)) was constructed. The presence of flagella increased invasiveness for chicken kidney cells (CKC) while its presence did not alter survival in HD11 macrophages. SG Fla(+) induced higher levels of CXCLi2, IL-6 and iNOS mRNA expression in CKC than the SG parent strain. The expression of genes responsible for immune response mediators in infected HD11 macrophages were not related to the presence of flagella. Mortality rates were lower in birds challenged with SG Fla(+) when compared with the SG parent. SG Fla(+) was recovered from caecal contents which showed pathological changes suggestive of inflammation and suggested increased colonization ability. (c) 2013 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 07/59233-3 - Importância do flagelo para a Patogenicidade de Salmonella enterica subespécie enterica sorovar Gallinarum biovar Gallinarum
Beneficiário:Oliveiro Caetano de Freitas Neto
Linha de fomento: Bolsas no Brasil - Doutorado