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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Peripheral kappa and delta opioid receptors are involved in the antinociceptive effect of crotalphine in a rat model of cancer pain

Texto completo
Autor(es):
Brigatte, Patricia [1, 2] ; Konno, Katsuhiro [3] ; Gutierrez, Vanessa Pacciari [1, 2] ; Sampaio, Sandra Coccuzzo [2, 1] ; Zambelli, Vanessa Olzon [2, 1] ; Picolo, Gisele [2, 1] ; Curi, Rui [4] ; Cury, Yara [2, 1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Inst Butantan, Lab Especial Dor & Sinalizacao, BR-05503900 Sao Paulo - Brazil
[2] Inst Butantan, Lab Fisiopatol, BR-05503900 Sao Paulo - Brazil
[3] Inst Butantan, Ctr Toxinol Aplicada, BR-05503900 Sao Paulo - Brazil
[4] Univ Sao Paulo, Inst Ciencias Biomed, Dept Fisiol & Biofis, BR-05508900 Sao Paulo - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Pharmacology Biochemistry and Behavior; v. 109, p. 1-7, AUG 2013.
Citações Web of Science: 8
Resumo

Cancer pain is an important clinical problem and may not respond satisfactorily to the current analgesic therapy. We have characterized a novel and potent analgesic peptide, crotalphine, from the venom of the South American rattlesnake Crotalus durissus terrificus. In the present work, the antinociceptive effect of crotalphine was evaluated in a rat model of cancer pain induced by intraplantar injection of Walker 256 carcinoma cells. Intraplantar injection of tumor cells caused the development of hyperalgesia and allodynia, detected on day 5 after tumor cell inoculation. Crotalphine (6 mu g/kg), administered p.o., blocked both phenomena. The antinociceptive effect was detected 1 h after treatment and lasted for up to 48 h. Intraplantar injection of nor-binaltorphimine (50 g/paw), a selective antagonist of kappa-opioid receptors, antagonized the antinociceptive effect of the peptide, whereas N,N-diallyl-Tyr-Aib-Phe-Leu (ICI 174,864, 10 mu g/paw), a selective antagonist of delta-opioid receptors, partially reversed this effect. On the other hand, D-Phe-Cys-Tyr-D-Trp-Orn-Thr-Pen-Thr amide (CTOP, 20 g/paw), an antagonist of mu-opioid receptors, did not modify crotalphine-induced antinociception. These data indicate that crotalphine induces a potent and long lasting opioid-mediated antinociception in cancer pain. (C) 2013 Elsevier Inc. All rights reserved. (AU)

Processo FAPESP: 08/57898-0 - Instituto Nacional de Ciência e Tecnologia em Toxinas
Beneficiário:Osvaldo Augusto Brazil Esteves Sant'Anna
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 98/14307-9 - Center for Applied Toxinology
Beneficiário:Hugo Aguirre Armelin
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs