Development and Validation of Methods for the Extraction of Phenolic Acids from Plasma, Urine, and Liver and Analysis by UPLC-MS

Autor(es)

de Oliveira, Daniela M. ^[1] ; Pinto, Carolina B. ^[1] ; Sampaio, Geni R. ^[1] ; Yonekura, Lina ^[1] ; Catharino, Rodrigo R. ^[2] ; Bastos, Deborah H. M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Publ Hlth, Dept Nutr, BR-01246904 Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Sch Med Sci, INNOVARE Biomarkers Lab, BR-13083887 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Agricultural and Food Chemistry; v. 61, n. 25, p. 6113-6121, JUN 26 2013.
• Citações Web of Science: 9

Resumo

This study developed and validated a method for the extraction and determination of 11 phenolic acids in rat plasma, urine, and liver by ultraperformance liquid, chromatography-mass spectrometry (UPLC-MS). A system suitability test (instrumental linearity, area, and retention time precision) was performed and recovery, intraday and between-day precisions, detection limits (LOD), and quantification limits (LOQ) were determined for all compounds in each biological matrix. Recoveries varied between 88 and 117% in plasma, between 87 and 102% in urine, and between 38 and 100% in liver. Precision was higher than 13.7% intraday and 14.0% interday in all matrices, at three concentration levels. To demonstrate the applicability, the method was used to estimate the concentrations of phenolic acids in samples from animals that received 5-caffeoylquinic acid (5-CQA) by gavage. The excellent validation results and the applicability of the method to real samples confirmed the suitability for studies on absorption, bioavailability, and pharmacokinetics of phenolic acids derived from foods rich in phenolic compounds. (AU)

• Processo FAPESP: 08/10308-4 - Bioacessibilidade, biodisponibilidade e atividade antioxidante de compostos fenólicos do Guaraná (Paullinia cupana) in vitro e in vivo
• Beneficiário: Lina Yonekura
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 09/01275-8 - Estudo da biodisponibilidade de compostos fenólicos da erva mate (Ilex paraguariensis) in vitro e in vivo.
• Beneficiário: Daniela Moura de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/14853-0 - Estudo da biodisponibilidade dos compostos fenólicos da erva mate (Ilex paraguariensis)
• Beneficiário: Deborah Helena Markowicz Bastos
• Modalidade de apoio: Auxílio à Pesquisa - Regular