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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Mesenchymal bone marrow stem cells within polyglycolic acid tube observed in vivo after six weeks enhance facial nerve regeneration

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Autor(es):
Zabeu Rossi Costa, Heloisa Juliana [1] ; Bento, Ricardo Ferreira [1] ; Salomone, Raquel [1] ; Azzi-Nogueira, Deborah [2] ; Zanatta, Daniela B. [3] ; Costa, Marcio Paulino [4] ; da Silva, Ciro Ferreira [5] ; Strauss, Bryan E. [3] ; Haddad, Luciana A. [2]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo Med Sch, Dept Otorhinolaryngol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Biosci Inst, Dept Genet & Evolutionary Biol, Sao Paulo - Brazil
[3] Univ Sao Paulo Med Sch, Inst Heart, Mol Cardiol Lab LIM13, Viral Vector Lab, Sao Paulo - Brazil
[4] Univ Sao Paulo Med Sch, Dept Plast Surg, Sao Paulo - Brazil
[5] Univ Sao Paulo, Dept Cell & Dev Biol, Inst Biomed Sci, Sao Paulo - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Brain Research; v. 1510, p. 10-21, MAY 13 2013.
Citações Web of Science: 27
Resumo

Autografting is the gold-standard method for facial nerve repair with tissue loss. Its association with high-quality scaffolds and cell implants has disclosed distinct experimental outcomes. The aim of this study was to evaluate the functional and histological effects of bone marrow stem cells (BMSC) combined with polyglycolic acid tube (PGAt) in autografted rat facial nerves. After neurotmesis of the mandibular branch of the rat facial nerve, surgical repair consisted of nerve autografting (groups A-E) contained in pGAT (groups B-E), filled with basement membrane matrix (groups C-E) with undifferentiated BMSC (group D) or Schwann-like cells that had differentiated from BMSC (group E). Axon morphometrics and an objective-compound muscle action potentials (CMAP) analysis were conducted. Immunofluorescence assays were carried out with Schwann cell marker S100 and anti-beta-galactosidase to label exogenous cells. Six weeks after surgery, animals from either cell-containing group had mean CMAP amplitudes significantly higher than control groups. Differently from other groups, facial nerves with Schwann-like cell implants had mean axonal densities within reference values. This same group had the highest mean axonal diameter in distal segments. We observed expression of the reporter gene lacZ in nerve cells in the graft and distally from it in groups D and E. Group-E cells had lacZ coexpressed with S100. In conclusion, regeneration of the facial nerve was improved by BMSC within PGAt in rats, yet Schwann-like cells were associated with superior effects. Accordingly, groups D and E had BMSC integrated in neural tissue with maintenance of former cell phenotype for six weeks. (C) 2013 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 98/14254-2 - Centro de Estudos do Genoma Humano
Beneficiário:Mayana Zatz
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 08/00972-4 - Tubo de ácido poliglicólico e células tronco derivadas da medula óssea diferenciadas in vitro na regeneração do nervo facial: estudo experimental em ratos
Beneficiário:Ricardo Ferreira Bento
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 08/53857-8 - Splicing alternativo e diversidade funcional do gene Fmr1
Beneficiário:Luciana Amaral Haddad
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 08/00584-4 - Tubo de ácido poliglicólico e células-tronco derivadas da medula óssea diferenciadas in vitro na regeneração do nervo facial: estudo experimental em ratos
Beneficiário:Heloísa Juliana Zabeu Rossi Costa
Linha de fomento: Bolsas no Brasil - Pós-Doutorado