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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Dengue Virus Type 3 Adaptive Changes during Epidemics in Sao Jose de Rio Preto, Brazil, 2006-2007

Texto completo
Autor(es):
Villabona-Arenas, Christian Julian [1] ; Mondini, Adriano [2] ; Bosch, Irene [3] ; Schimitt, Diane [4] ; Calzavara-Silva, Carlos E. [5] ; Zanotto, Paolo M. de A. [1] ; Nogueira, Mauricio L. [6]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, LEMB, BR-05508 Sao Paulo - Brazil
[2] Univ Estadual Paulista Julio de Mesquita Filho Ar, Fac Ciencias Farmaceut, Dept Ciencias Biol, Lab Saude Publ, Sao Paulo - Brazil
[3] MIT, Div Hlth Sci & Technol, Cambridge, MA 02139 - USA
[4] Tufts Univ, Dept Infect Dis & Global Hlth, Cummings Sch Vet Med, North Grafton, MA - USA
[5] Fundacao Oswaldo Cruz Fiocruz, Ctr Pesquisas Rene Rachou CPqRR, LICM, Belo Horizonte, MG - Brazil
[6] Fac Med Sao Jose do Rio Preto, Lab Pesquisas Virol, Sao Jose Do Rio Preto, SP - Brazil
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 8, n. 5 MAY 7 2013.
Citações Web of Science: 7
Resumo

Global dengue virus spread in tropical and sub-tropical regions has become a major international public health concern. It is evident that DENV genetic diversity plays a significant role in the immunopathology of the disease and that the identification of polymorphisms associated with adaptive responses is important for vaccine development. The investigation of naturally occurring genomic variants may play an important role in the comprehension of different adaptive strategies used by these mutants to evade the human immune system. In order to elucidate this role we sequenced the complete polyprotein-coding region of thirty-three DENV-3 isolates to characterize variants circulating under high endemicity in the city of Sao Jose de Rio Preto, Brazil, during the onset of the 2006-07 epidemic. By inferring the evolutionary history on a local-scale and estimating rates of synonymous (dS) and nonsynonimous (dN) substitutions, we have documented at least two different introductions of DENV-3 into the city and detected 10 polymorphic codon sites under significant positive selection (dN/dS > 1) and 8 under significant purifying selection (dN/dS < 1). We found several polymorphic amino acid coding sites in the envelope (15), NS1 (17), NS2A ( 11), and NS5 (24) genes, which suggests that these genes may be experiencing relatively recent adaptive changes. Furthermore, some polymorphisms correlated with changes in the immunogenicity of several epitopes. Our study highlights the existence of significant and informative DENV variability at the spatio-temporal scale of an urban outbreak. (AU)

Processo FAPESP: 10/19059-7 - Filogeografia do vírus da dengue nos municípios de Jundiaí e Guarujá no estado de São Paulo
Beneficiário:Paolo Marinho de Andrade Zanotto
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 12/11733-6 - Caracterização biológica e molecular dos isolados de dengue, sorotipo 1 e 2, em São José do Rio Preto, SP, detectados nas temporadas de 2006 a 2013
Beneficiário:Maurício Lacerda Nogueira
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 11/17071-2 - Filogeografia do vírus da dengue nos municípios de Jundiaí e Guarujá no estado de São Paulo
Beneficiário:Christian Julián Villabona Arenas
Linha de fomento: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 11/10458-9 - Vigilância de dengue: avaliação de um sistema sentinela e estudos moleculares dos sorotipos de dengue
Beneficiário:Maurício Lacerda Nogueira
Linha de fomento: Auxílio à Pesquisa - Regular