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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

The influence of sleep deprivation and obesity on DNA damage in female Zucker rats

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Autor(es):
Tenorio, Neuli M. [1] ; Ribeiro, Daniel A. [2] ; Alvarenga, Tathiana A. [1] ; Fracalossi, Ana Carolina C. [2] ; Carlin, Viviane [2] ; Hirotsu, Camila [1] ; Tufik, Sergio [1] ; Andersen, Monica L. [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Fed Sao Paulo UNIFESP, Dept Psicobiol, Sao Paulo - Brazil
[2] Univ Fed Sao Paulo UNIFESP, Dept Biociencia, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Clinics; v. 68, n. 3, p. 385-389, 2013.
Citações Web of Science: 7
Resumo

OBJECTIVE: The aim of this study was to evaluate overall genetic damage induced by total sleep deprivation in obese, female Zucker rats of differing ages. METHOD: Lean and obese Zucker rats at 3, 6, and 15 months old were randomly distributed into two groups for each age group: home-cage control and sleep-deprived (N = 5/group). The sleep-deprived groups were deprived sleep by gentle handling for 6 hours, whereas the home-cage control group was allowed to remain undisturbed in their home-cage. At the end of the sleep deprivation period, or after an equivalent amount of time for the home-cage control groups, the rats were brought to an adjacent room and decapitated. The blood, brain, and liver tissue were collected and stored individually to evaluate DNA damage. RESULTS: Significant genetic damage was observed only in 15-month-old rats. Genetic damage was present in the liver cells from sleep-deprived obese rats compared with lean rats in the same condition. Sleep deprivation was associated with genetic damage in brain cells regardless of obesity status. DNA damage was observed in the peripheral blood cells regardless of sleep condition or obesity status. CONCLUSION: Taken together, these results suggest that obesity was associated with genetic damage in liver cells, whereas sleep deprivation was associated with DNA damage in brain cells. These results also indicate that there is no synergistic effect of these noxious conditions on the overall level of genetic damage. In addition, the level of DNA damage was significantly higher in 15-month-old rats compared to younger rats. (AU)

Processo FAPESP: 09/03360-2 - Biomonitoramento citogenético em usuários de enxaguatórios bucais: análises in vitro e in vivo
Beneficiário:Viviane Carlin
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 98/14303-3 - Center for Sleep Studies
Beneficiário:Sergio Tufik
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 11/12325-6 - Efeitos da privação de sono e da síndrome da apnéia obstrutiva do sono na função reprodutiva em homens
Beneficiário:Tathiana Aparecida Fernandes de Alvarenga
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 07/01228-4 - Carcinogênese bucal quimicamente induzida pela 4-nitroquinolina 1-óxido em ratos: possíveis biomarcadores envolvidos em sua patogênese
Beneficiário:Daniel Araki Ribeiro
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 10/50129-1 - Insuficiência renal crônica, restrição de sono e sildenafil: consequências renais, cardíacas e sexuais em um modelo animal
Beneficiário:Camila Hirotsu
Linha de fomento: Bolsas no Brasil - Doutorado Direto