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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Use of electrospray tandem mass spectrometry for identification of microcystins during a cyanobacterial bloom event

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Autor(es):
Frias, Humberto Vieira ; Mendes, Maria Anita ; Cardozo, Karina Helena Morais ; Carvalho, Valdemir Melechco ; Tomazela, Daniela ; Colepicolo, Pio ; Pinto, Ernani [7]
Número total de Autores: 7
Tipo de documento: Artigo Científico
Fonte: Biochemical and Biophysical Research Communications; v. 344, n. 3, p. 741-746, June 2006.
Área do conhecimento: Ciências da Saúde - Farmácia
Assunto(s):Cianobactérias   Microcistinas   Reservatórios   Represas   Espectrometria de massas em Tandem
Resumo

Drastic environmental conditions such as elevated temperature, abrupt pH variation, low turbulence, and high nutrient inputs can enhance the development of toxic cyanobacterial blooms in lakes and reservoirs. This study describes the occurrence of four microcystin variants (MC) in a bloom in the eutrophic reservoir Billings, in São Paulo City. The bloom sample was collected in October 2003, and Microcystis were the main genus found. The MC were separated and purified by reverse phase high performance liquid chromatography (RP-HPLC). Their structures were elucidated by electrospray ionization tandem mass spectrometry (ESI-MS/MS) and four MC variants were determined: MC-RR, MC-LR, MC-YR, and MC-hRhR. MC-hRhR is described for the first time as a new variant of MC with two homoarginines at positions 2 and 4 in its structure. ESI-MS/MS analysis thus provides a powerful and convenient tool for the determination of variants of MC. These results represent an important contribution to the knowledge of the biochemistry of toxic cyanobacteria and their toxins, specifically in São Paulo State. (AU)

Processo FAPESP: 04/14264-0 - Avaliação dos possíveis mecanismos de ação das microcistinas sobre a função de neutrófilos humanos
Beneficiário:Ernani Pinto Junior
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 03/06443-0 - Algas presentes em florações: isolamento e padronização de métodos analíticos para a quantificação das toxinas
Beneficiário:Ernani Pinto Junior
Linha de fomento: Auxílio à Pesquisa - Pesquisa em Políticas Públicas