Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Genomic divergence of zebu and taurine cattle identified through high-density SNP genotyping

Texto completo
Autor(es):
Porto-Neto, Laercio R. [1, 2] ; Sonstegard, Tad S. [3] ; Liu, George E. [3] ; Bickhart, Derek M. [3] ; Da Silva, Marcos V. B. [4] ; Machado, Marco A. [4] ; Utsunomiya, Yuri T. [5] ; Garcia, Jose F. [5] ; Gondro, Cedric [2] ; Van Tassell, Curtis P. [3]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ Queensland, Sch Vet Sci, Anim Genet Lab, Gatton, Qld 4343 - Australia
[2] Univ New England, Sch Environm & Rural Sci, Armidale, NSW 2351 - Australia
[3] ARS, USDA, Bovine Funct Genom Lab, Beltsville, MD 20705 - USA
[4] Embrapa Dairy Cattle, Bioinformat & Anim Genom Lab, Juiz De Fora, MG - Brazil
[5] Univ Estadual Paulista, UNESP, Aracatuba, SP - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: BMC Genomics; v. 14, DEC 13 2013.
Citações Web of Science: 54
Resumo

Background: Natural selection has molded evolution across all taxa. At an arguable date of around 330,000 years ago there were already at least two different types of cattle that became ancestors of nearly all modern cattle, the Bos taurus taurus more adapted to temperate climates and the tropically adapted Bos taurus indicus. After domestication, human selection exponentially intensified these differences. To better understand the genetic differences between these subspecies and detect genomic regions potentially under divergent selection, animals from the International Bovine HapMap Experiment were genotyped for over 770,000 SNP across the genome and compared using smoothed F-ST. The taurine sample was represented by ten breeds and the contrasting zebu cohort by three breeds. Results: Each cattle group evidenced similar numbers of polymorphic markers well distributed across the genome. Principal components analyses and unsupervised clustering confirmed the well-characterized main division of domestic cattle. The top 1% smoothed F-ST, potentially associated to positive selection, contained 48 genomic regions across 17 chromosomes. Nearly half of the top F-ST signals (n = 22) were previously detected using a lower density SNP assay. Amongst the strongest signals were the BTA7:similar to 50 Mb and BTA14:similar to 25 Mb; both regions harboring candidate genes and different patterns of linkage disequilibrium that potentially represent intrinsic differences between cattle types. The bottom 1% of the smoothed F-ST values, potentially associated to balancing selection, included 24 regions across 13 chromosomes. These regions often overlap with copy number variants, including the highly variable region at BTA23:similar to 24 Mb that harbors a large number of MHC genes. Under these regions, 318 unique Ensembl genes are annotated with a significant overrepresentation of immune related pathways. Conclusions: Genomic regions that are potentially linked to purifying or balancing selection processes in domestic cattle were identified. These regions are of particular interest to understand the natural and human selective pressures to which these subspecies were exposed to and how the genetic background of these populations evolved in response to environmental challenges and human manipulation. (AU)

Processo FAPESP: 10/52030-2 - Estudos de associação genômica das características reprodutivas de touros zebuínos (Bos indicus) utilizando SNP chip de alta densidade
Beneficiário:José Fernando Garcia
Linha de fomento: Auxílio à Pesquisa - Regular