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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Comparison of lipase production on crambe oil and meal by Fusarium sp (Gibberella fujikuroi complex)

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Autor(es):
Oliveira, Bruno H. [1] ; Coradi, Gilberto V. [1] ; Attili-Angelis, Derlene [2] ; Scauri, Carolina [1] ; Luques, Ana Helena P. G. [1] ; Barbosa, Aneli M. [3] ; Dekker, Robert F. H. [3] ; Oliva Neto, Pedro [1] ; Lima, Valeria M. G. [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, UNESP, Dept Ciencias Biol, Lab Biotecnol Ind, Fac Ciencias & Letras, BR-19806900 Assis, SP - Brazil
[2] Univ Estadual Campinas, Ctr Pluridisciplinar Pesquisas Quim Biol & Agr CP, Div Recursos Microbianos, Campinas, SP - Brazil
[3] Lakehead Univ, Biorefining Res Inst, Thunder Bay, ON P7B 5E1 - Canada
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY; v. 115, n. 12, SI, p. 1413-1425, DEC 2013.
Citações Web of Science: 4
Resumo

This study provides a comparison of fermentation conditions for the production lipases by Fusarium sp. (Gibberella fujikuroi complex) isolate FCLA-MA-41 in submerged (SmF) and solid-state fermentation (SSF) using agro-industrial residues. To assess SmF, a univariate study of seven carbon sources (crambe, corn, linseed, olive, palm (dende), soybean oils and chicken fat) and four nitrogen sources (ammonium sulfate, sodium nitrate, urea, yeast extract) was performed. The relationship among the concentrations of carbon and nitrogen sources, Triton X-100 and yeast extract was studied using a Central Composite Design (CCD). To assess SSF, different residues (sugarcane bagasse) and oil-seed meals (castorbean, corn, crambe, soybean) with additional supplements added (minimum salts, seed oils) were examined. Based on the CCD matrix, a medium containing crambe oil (17.5mL/L), Triton X-100 (5g/L), ammonium sulfate (5g/L) and yeast extract (1g/L) was proposed, resulting in a lipase titer of 3.0 +/- 0.25U/mL. The cost/production relationship was 7.73% less expensive than SmF with corn oil. SSF produced a maximum lipase titer of 5.0 +/- 0.25U/gds on crambe meal moistened with phosphate buffer. The best cost/enzyme activity estimate was for SSF with crambe meal as substrate in only distilled water (87.27% less expensive than SmF). (AU)

Processo FAPESP: 10/07998-9 - Transesterificação de óleos vegetais pela lipase imobilizada de Fusarium sp. produzida por fermentação submersa e por fermentação em estado sólido
Beneficiário:Valéria Marta Gomes de Lima
Linha de fomento: Auxílio à Pesquisa - Regular