Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Comparison of the hemolytic activity between C. albicans and non-albicans Candida species

Texto completo
Autor(es):
Rossoni, Rodnei Dennis [1] ; Barbosa, Junia Oliveira [1] ; Godinho Vilela, Simone Furgeri [1] ; Cardoso Jorge, Antonio Olavo [1] ; Junqueira, Juliana Campos [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista UNESP, Inst Sci & Technol, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Brazilian Oral Research; v. 27, n. 6, p. 484-489, NOV-DEC 2013.
Citações Web of Science: 25
Resumo

The ability to produce enzymes, such as hemolysins, is an important virulence factor for the genus Candida. The objective of this study was to compare the hemolytic activity between C. albicans and non-albicans Candida species. Fifty strains of Candida species, isolated from the oral cavity of patients infected with HIV were studied. The isolates included the following species: C. albicans, C. dubliniensis, C. glabrata, C. tropicalis, C. krusei, C. parapsilosis, C. dubliniensis, C. norvegensis, C. lusitaniae, and C. guilliermondii. Hemolysin production was evaluated on Sabouraud dextrose agar containing chloramphenicol, blood, and glucose. A loop-full of pure Candida culture was spot-inoculated onto plates and incubated at 37 degrees C for 24 h in a 5% CO2 atmosphere. Hemolytic activity was defined as the formation of a translucent halo around the colonies. All C. albicans strains that were studied produced hemolysins. Among the non-albicans Candida species, 86% exhibited hemolytic activity. Only C. guilliermondii and some C. parapsilosis isolates were negative for this enzyme. In conclusion, most non-albicans Candida species had a similar ability to produce hemolysins when compared to C. albicans. (AU)

Processo FAPESP: 12/02184-9 - Interações entre diferentes espécies de Candida na candidose experimental em modelos de invertebrados e vertebrados
Beneficiário:Juliana Campos Junqueira
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 07/54442-3 - Efeitos bactericidas do verde de malaquita e azuleno associados ao laser de baixa intensidade sobre Enterobacteriaceae
Beneficiário:Rodnei Dennis Rossoni
Linha de fomento: Bolsas no Brasil - Iniciação Científica