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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

West Nile virus surveillance, Brazil, 2008-2010

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Autor(es):
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Ometto, Tatiana [1] ; Durigon, Edison Luiz [1] ; de Araujo, Jansen [1] ; Aprelon, Rosalie [2] ; de Aguiar, Daniel Moura [3] ; Cavalcante, Guacyara Tenorio [4] ; Melo, Rosane Marini [5] ; Levi, Jose Eduardo [6] ; de Azevedo Junior, Severino Mendes [7] ; Petry, Maria Virginia [8] ; Neto, Isaac Simao [9] ; Serafini, Patricia [9] ; Villalobos, Eliana [10] ; Sequetin Cunha, Elenice Maria [10] ; Lara, Maria do Carmo Custodio S. H. [10] ; Dales Nava, Alessandra Ferreira [11] ; Nardi, Marcello Schiavo [12] ; Hurtado, Renata [13, 1] ; Rodrigues, Roberta [7] ; Sherer, Angelo Luis [8] ; Martins Sherer, Janete de Fatima [8] ; Geraldi, Marcelo Plaisant [6] ; Moraes de Seixas, Marina Maria [1] ; Peterka, Cassio [12] ; Bandeira, Debora de Souza [12] ; Pradel, Jennifer [2] ; Vachiery, Nathalie [2] ; Labruna, Marcelo Bahia [13] ; Aranha de Camargo, Luiz Marcelo [14] ; Lanciotti, Robert [15] ; Lefrancois, Thierry [2]
Número total de Autores: 31
Afiliação do(s) autor(es):
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[1] Univ Sao Paulo, Lab Virol Clin & Mol BSL3, ICB, BR-05508900 Sao Paulo - Brazil
[2] CIRAD UMR CMAEE CIRAD INRA, Petit Bourg 97170 - Guadeloupe
[3] Univ Fed Mato Grosso UFMT, Dept Clin Med Vet, BR-78060900 Cuiaba - Brazil
[4] Univ Sao Paulo, Inst Fis, BR-05508090 Sao Paulo - Brazil
[5] Inst Defesa Agr Estado Mato Gross INDEA, BR-78050970 Cuiaba - Brazil
[6] Univ Sao Paulo, Virol Lab, Inst Med Trop Sao Paulo, BR-05403000 Sao Paulo - Brazil
[7] Univ Fed Rural Pernambuco UFRPE, BR-52171900 Recife, PE - Brazil
[8] Univ Vale Rio Sinos UNISINOS, BR-93022000 Sao Leopoldo - Brazil
[9] Ctr Nacl Pesquisa & Conservacao Aves Silvestre CE, BR-88053700 Florianopolis, SC - Brazil
[10] Inst Biol, Ctr Pesquisa & Desenvolvimento Sanidade Anim, BR-04014002 Sao Paulo - Brazil
[11] EcoHlth Alliance, BR-05508270 Sao Paulo - Brazil
[12] IPE, BR-19280000 Teodoro Sampaio - Brazil
[13] Univ Sao Paulo, Fac Med Vet & Zootecnia, BR-05508270 Sao Paulo - Brazil
[14] Univ Sao Paulo, Inst Ciencias Biomed 5, BR-78965300 Monte Negro - Brazil
[15] Ctr Dis Control & Prevent CDC, Ft Collins, CO 80521 - USA
Número total de Afiliações: 15
Tipo de documento: Artigo Científico
Fonte: Transactions of the Royal Society of Tropical Medicine and Hygiene; v. 107, n. 11, p. 723-730, NOV 2013.
Citações Web of Science: 23
Resumo

Background: West Nile virus (WNV) is an emergent pathogen that is widely distributed in North and Central America. The recent introduction in South America has focused attention on the spread of WNV across Southern American countries. The transmission network involves mosquitoes, birds, horses and humans. Methods: The serological evaluation of sera from 678 equids and 478 birds was performed using a WNV-specific blocking ELISA, and only the positive results were confirmed by plaque reduction neutralisation tests (PRNTs). Molecular analysis was performed on sera from 992 healthy equids and on 63 macerates of brains from equids that died of encephalitis and had previously tested negative for other pathogens. We also tested swabs from 928 birds. The samples analysed were collected in different biomes of Brazil. Results: We identified WNV antibodies by ELISA in thirteen equids and five birds, and PRNT90 confirmed WNV positivity in four equid samples collected in 2009 in an area between the Amazon and the Pantanal. None of the ELISA positive bird samples were confirmed by PRNT90, and all samples tested by RT-PCR were negative. Conclusion: WNV circulation is confirmed by this large scale survey even in the absence of detection of clinical cases. (AU)

Processo FAPESP: 09/05994-9 - Monitoramento do Vírus do Oeste do Nilo no Brasil
Beneficiário:Tatiana Ometto de Araujo
Linha de fomento: Bolsas no Brasil - Doutorado Direto