Current Status of Research on Fungal Bioluminescence: Biochemistry and Prospects for Ecotoxicological Application

Autor(es)

Stevani, Cassius V. ^[1] ; Oliveira, Anderson G. ^[2] ; Mendes, Luiz F. ^[3] ; Ventura, Fernanda F. ^{[1, 4]} ; Waldenmaier, Hans E. ^{[1, 3]} ; Carvalho, Rodrigo P. ^[1] ; Pereira, Tatiana A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, Sao Paulo - Brazil

^[2] Univ Fed Sao Carlos, Dept Genet & Evolucao, Sorocaba, SP - Brazil

^[3] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo - Brazil

^[4] Fundacao Jorge Duprat Figueiredo Seguranca & Med, Sao Paulo - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: Photochemistry and Photobiology; v. 89, n. 6, p. 1318-1326, NOV 2013.
• Citações Web of Science: 15

Resumo

Over the last half decade the study of fungal bioluminescence has regained momentum since the involvement of enzymes has been confirmed after over 40years of controversy. Since then our laboratory has worked mainly on further characterizing the substances involved in fungal bioluminescence and its mechanism, as well as the development of an ecotoxicological bioluminescent assay with fungi. Previously, we proved the involvement of a NAD(P)H-dependent reductase and a membrane-bound luciferase in a two-step reaction triggered by addition of NAD(P)H and molecular oxygen to generate green light. The fungal luminescent system is also likely shared across all lineages of bioluminescent fungi based on cross-reaction studies. Moreover, fungal bioluminescence is inhibited by the mycelium exposure to toxicants. The change in light emission under optimal and controlled conditions has been used as endpoint in the development of toxicological bioassays. These bioassays are useful to better understand the interactions and effects of hazardous compounds to terrestrial species and to assist the assessment of soil contaminations by biotic or abiotic sources. In this work, we present an overview of the current state of the study of fungal luminescence and the application of bioluminescent fungi as versatile tool in ecotoxicology. (AU)

• Processo FAPESP: 04/13856-1 - Estudo mecanístico da bioluminescência do fungo Gerronema viridilucens
• Beneficiário: Anderson Garbuglio de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 09/54718-4 - Efeitos de metais mono-, bi e trivalentes sobre a fisiologia de algas
• Beneficiário: Luiz Fernando Mendes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 01/04753-6 - Bioluminescência e atividade farmacológica de cogumelos
• Beneficiário: Cassius Vinicius Stevani
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 10/15047-4 - Bioluminescência em fungos: prospecção, função biológica e ensaios toxicológicos
• Beneficiário: Cassius Vinicius Stevani
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/53628-3 - Estudo da bioluminescencia de fungos e suas aplicacoes em quimica ambiental.
• Beneficiário: Cassius Vinicius Stevani
• Modalidade de apoio: Auxílio à Pesquisa - Regular