Influence of bovine sperm DNA fragmentation and oxidative stress on early embryo in vitro development outcome

Autor(es)

Simoes, Renata ^{[1, 2]} ; Feitosa, Weber Beringui ^[1] ; Perez Siqueira, Adriano Felipe ^[1] ; Nichi, Marcilio ^[1] ; Paula-Lopes, Fabiola Freitas ^[3] ; Marques, Mariana Groke ^[4] ; Peres, Maria Angelica ^[5] ; Barnabe, Valquiria Hyppolito ^[1] ; Visintin, Jose Antonio ^[1] ; Ortiz Assumpcao, Mayra Elena ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Coll Vet Med, Dept Anim Reprod, BR-05508270 Sao Paulo - Brazil

^[2] Fed Univ ABC, Ctr Ciencias Nat & Humanas, BR-09210170 Santo Andre, SP - Brazil

^[3] Univ Fed Sao Paulo, Dept Biol Sci, BR-09972270 Diadema, SP - Brazil

^[4] Embrapa Suinos & Aves, EMBRAPA Swine & Poultry Res Ctr, BR-89700000 Concordia, SC - Brazil

^[5] Univ Sao Paulo, Fac Med, Dept Patol, Lab Poluicao Atmosfer Expt LIM05, BR-01246903 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Reproduction; v. 146, n. 5, p. 433-441, NOV 2013.
• Citações Web of Science: 43

Resumo

Sperm chromatin fragmentation may be caused by a number of factors, the most significant of which is reactive oxygen species. However, little is known about the effect of sperm oxidative stress (OS) on DNA integrity, fertilization, and embryonic development in cattle. Therefore, the goal of this study was to evaluate the influence of sperm OS susceptibility on the DNA fragmentation rate and in vitro embryo production (IVP) in a population of bulls. Groups of cryopreserved sperm samples were divided into four groups, based on their susceptibility to OS (G1, low OS; G2, average OS; G3, high OS; and G4, highest OS). Our results demonstrated that the sperm DNA integrity was compromised in response to increased OS susceptibility. Furthermore, semen samples with lower susceptibility to OS were also less susceptible to DNA damage (G1, 4.06%; G2, 6.09%; G3, 6.19%; and G4, 6.20%). In addition, embryo IVP provided evidence that the embryo cleavage rate decreased as the OS increased (G1, 70.18%; G2, 62.24%; G3, 55.85%; and G4, 50.93%), but no significant difference in the blastocyst rate or the number of blastomeres was observed among the groups. The groups with greater sensitivity to OS were also associated with a greater percentage of apoptotic cells (G1, 2.6%; G2, 2.76%; G3, 5.59%; and G4, 4.49%). In conclusion, we demonstrated that an increased susceptibility to OS compromises sperm DNA integrity and consequently reduces embryo quality. (AU)

• Processo FAPESP: 07/55294-8 - Influencia dos indices espermaticos de protamina sobre a fertilidade in vitro em bovinos.
• Beneficiário: Renata Simões
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 07/58487-1 - Influencia dos indices espermaticos de protamina sobre a fertilidade in vitro em bovinos.
• Beneficiário: Mayra Elena Ortiz D' Avila Assumpcao
• Modalidade de apoio: Auxílio à Pesquisa - Regular