Methylation Pattern of THBS1, GATA-4, and HIC1 in Pediatric and Adult Patients Infected with Helicobacter pylori

Autor(es)

Alvarez, Marisa Claudia ^{[1, 2]} ; Placido Ladeira, Marcelo Sady ^[3] ; Affonso Scaletsky, Isabel Cristina ^[4] ; Pedrazzoli, Jr., Jose ^[1] ; Ribeiro, Marcelo Lima ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Francisco, Unidade Integrada Farmacol & Gastroenterol, BR-12900000 Braganca Paulista, SP - Brazil

^[2] Univ Estadual Campinas, Programa Posgrad Genet & Biol Mol, Campinas, SP - Brazil

^[3] UNESP, Dept Clin Med, Botucatu, SP - Brazil

^[4] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Digestive Diseases and Sciences; v. 58, n. 10, p. 2850-2857, OCT 2013.
• Citações Web of Science: 10

Resumo

Background Helicobacter pylori infection is usually acquired in childhood and persists into adulthood if untreated. The bacterium induces a chronic inflammatory response, which is associated with epigenetic alterations in oncogenes, tumor-suppressor genes, cell-cycle regulators, and cell-adhesion molecules. Aim The aim of this study was to analyze the effect of H. pylori infection on the methylation status of Thrombospondin-1 (THBS1), Hypermethylated in cancer 1 (HIC1) and Gata binding protein-4 (GATA-4) in gastric biopsy samples from children and adults infected or uninfected with the bacterium and in samples obtained from gastric cancer patients. Methods The methylation pattern was analyzed with methylation-specific PCR. Results Our results showed that H. pylori infection was associated with methylation of the promoter regions of the THBS1 and GATA-4 genes in pediatric and adult samples (p < 0.01). HIC1 showed the lowest level of methylation, which was not an early event during gastric carcinogenesis. Conclusions The results from this study indicate that methylation of THBS1 and GATA-4 occurs in the early stages of chronic gastritis and gastric cancer in association with H. pylori infection; however, in gastric cancer samples, other mechanisms cooperate with the down-regulation of these genes. Methylation of HIC1 may not be the principal mechanism implicated in its down-regulation in gastric cancer samples. (AU)

• Processo FAPESP: 08/02678-6 - ANÁLISE DA INFLUÊNCIA DO Helicobacter pylori NO PADRÃO DE METILAÇÃO DE GENES RELACIONADOS AO MECANISMO DE REPARO AO DNA E NA INSTABILIDADE DE MICROSSATÉLITES
• Beneficiário: Marisa Claudia Alvarez de Prax
• Modalidade de apoio: Bolsas no Brasil - Doutorado