Orthodontic Force Increases Interleukin-1 beta and Tumor Necrosis Factor-alpha Expression and Alveolar Bone Loss in Periodontitis

Autor(es)

Boas Nogueira, Andressa Vilas ^[1] ; Chaves de Souza, Joao Antonio ^[1] ; Kim, Yeon Jung ^[2] ; de Sousa-Neto, Manoel Damiao ^[3] ; Cirelli, Carolina Chan ; Cirelli, Joni Augusto ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Sch Dent Araraquara, Dept Diag & Surg, Araraquara - Brazil

^[2] Univ Santo Amaro, Dept Implantol, Santo Amaro - Brazil

^[3] Univ Sao Paulo, Dept Restorat Dent, BR-14049 Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Periodontology; v. 84, n. 9, p. 1319-1326, SEP 2013.
• Citações Web of Science: 17

Resumo

Background: The present study aims to evaluate the effects of orthodontic movement (OM) on the periodontal tissues of rats with ligature-induced periodontal disease. Methods: Eighty-eight rats were divided into four groups: 1) negative control (sham operated); 2) periodontal disease; 3) OM; and 4) periodontal disease followed by OM (OMP). Rats were sacrificed 3 hours or 1, 3, or 7 days after OM commencement. Bone volume fraction (BVF) and bone mineral density (BMD) were assessed in hemimaxillae by microcomputed tomography analysis. Expression of the proinflammatory cytokines interleukin (IL)-1 beta and tumor necrosis factor (TNF)-alpha were evaluated in gingival samples by quantitative polymerase chain reaction and enzyme-linked immunosorbent assay, and in the furcation region by immunohistochemistry analysis (IHC). Results: The OMP group had lower BVF and BMD levels compared to the other groups at day 7 (P <0.05). Maximum messenger ribonucleic acid expression of both cytokines was observed in the OMP group at day 1 (P <0.05). In the same period, all proteins were expressed in high levels for all test groups compared to the control group. The number of cells positive for IL-1 beta and TNF-alpha by IHC was highest in the OMP group at day 1, with progressive reduction thereafter. Conclusion: The results suggest that OM acts synergistically with periodontal disease in periodontal breakdown through upregulation of proinflammatory cytokines. (AU)

• Processo FAPESP: 10/07771-4 - Efeito da movimentação ortodôntica na progressão da doença periodontal. Estudos in vivo e in vitro.
• Beneficiário: Andressa Vilas Boas Nogueira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 08/02506-0 - Efeitos da movimentação ortodôntica na expressão de mediadores inflamatórios nos tecidos periodontais de ratos em condições de saúde ou doença periodontal induzida.
• Beneficiário: Andressa Vilas Boas Nogueira
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 08/06328-0 - Efeitos da movimentação ortodôntica na expressão de mediadores inflamatórios nos tecidos periodontais de ratos em condições de saúde ou doença periodontal induzida
• Beneficiário: Joni Augusto Cirelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular