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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

BONE MARROW STEM CELLS IN FACIAL NERVE REGENERATION FROM ISOLATED STUMPS

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Autor(es):
Salomone, Raquel [1] ; Bento, Ricardo F. [1] ; Costa, Heloisa J. Z. R. [1] ; Azzi-Nogueira, Deborah [2] ; Ovando, Patricia C. [3] ; Da-Silva, Cira F. [4] ; Zanatta, Daniela B. [5] ; Strauss, Bryan E. [5] ; Haddad, Luciana A. [2]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Otorhinolaryngol, Sch Med, BR-05403000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Genet & Evolutionary Biol, Biosci Inst, BR-05403000 Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Dept Biochem, Sao Paulo - Brazil
[4] Univ Sao Paulo, Dept Cell & Dev Biol, Inst Biomed Sci, BR-05403000 Sao Paulo - Brazil
[5] Univ Sao Paulo, Viral Vector Lab, Mol Cardiol Lab LIM 13, Heart Inst, Med Sch, BR-05403000 Sao Paulo - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: MUSCLE & NERVE; v. 48, n. 3, p. 423-429, SEP 2013.
Citações Web of Science: 17
Resumo

IntroductionSevere lesions in the facial nerve may have extensive axonal loss and leave isolated stumps that impose technical difficulties for nerve grafting. Methods: We evaluated bone marrow stem cells (BMSC) in a silicone conduit for rat facial nerve regeneration from isolated stumps. Group A utilized empty silicone tubes; in groups B-D, the tube was filled with acellular gel; and, in groups C and D, undifferentiated BMSC (uBMSC) or Schwann-like cells differentiated from BMSC (dBMSC) were added, respectively. Compound muscle action potentials (CMAPs) were measured, and histology was evaluated. Results: Groups C and D had the highest CMAP amplitudes. Group C had shorter CMAP durations than groups A, B, and D. Distal axonal number and density were increased in group C compared with groups A and B. Conclusions: Regeneration of the facial nerve was improved by both uBMSC and dBMSC in rats, yet uBMSC was associated with superior functional results. (AU)

Processo FAPESP: 98/14254-2 - Centro de Estudos do Genoma Humano
Beneficiário:Mayana Zatz
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 08/00458-9 - Avaliação da regeneração do nervo facial de ratos após a implantação de células tronco derivadas do estroma de medula óssea diferenciadas in vitro
Beneficiário:Ricardo Ferreira Bento
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 08/53857-8 - Splicing alternativo e diversidade funcional do gene Fmr1
Beneficiário:Luciana Amaral Haddad
Linha de fomento: Auxílio à Pesquisa - Regular