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Identification of two novel cytolysins from the hydrozoan Olindias sambaquiensis (Cnidaria)

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Autor(es):
Haddad Junior, Vidal [1] ; Zara, Fernando [2] ; Marangoni, Sergio [3] ; Toyama, Daniela de Oliveira [4] ; Felizardo de Souza, Alex Jardelino [2] ; Buzzo de Oliveira, Simone Cristina [2, 1] ; Toyama, Marcos Hikari [2, 5]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, UNESP, Botucatu Med Sch, Sao Paulo State Univ, Botucatu, SP - Brazil
[2] Univ Estadual Paulista, UNESP, Sao Paulo State Univ, Sao Vicente, SP - Brazil
[3] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Biochem, Campinas, SP - Brazil
[4] Univ Prebiteriana Mackenzie, Ctr Biol & Hlth Sci, Sao Paulo - Brazil
[5] UNESP, Unidade Sao Vicente, BR-11330900 Sao Vicente, SP - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 20, p. 1-6, Mar. 2014.
Citações Web of Science: 9
Resumo

Background : Although the hydrozoan Olindias sambaquiensis is the most common jellyfish associated with human envenomation in southeastern and southern Brazil, information about the composition of its venom is rare. Thus, the present study aimed to analyze pharmacological aspects of O. sambaquiensis venom as well as clinical manifestations observed in affected patients. Crude protein extracts were prepared from the tentacles of animals; peptides and proteins were sequenced and submitted to circular dichroism spectroscopy. Creatine kinase, cytotoxicity and hemolytic activity were evaluated by specific methods. Results : We identified two novel cytolysins denominated oshem 1 and oshem 2 from the tentacles of this jellyfish. The cytolysins presented the amino acid sequences NEGKAKCGNTAGSKLTFKSADECTKTGQK (oshem 1) and NNSKAKCGDLAGWSKLTFKSADECTKTGQKS (oshem 2) with respective molecular masses of 3.013 kDa and 3.375 kDa. Circular dichroism revealed that oshem 1 has random coils and small α-helix conformation as main secondary structure whereas oshem 2 presents mainly random coils as its main secondary structure probably due to the presence of W (13) in oshem 2. The hemolysis levels induced by oshem 1 and oshem 2 using a peptide concentration of 0.2 mg/mL were, respectively, 51.7 ± 6.5% and 32.9 ± 8.7% (n = 12 and p ≤ 0.05). Oshem 1 and oshem 2 showed significant myonecrotic activity, evaluated by respective CK level measurements of 1890.4 ± 89 and 1212.5 ± 103 (n = 4 and p ≤ 0.05). In addition, myonecrosis was also evaluated by cell survival, which was measured at 72.4 ± 8.6% and 83.5 ± 6.7% (n = 12 and p ≤ 0.05), respectively. The structural analysis showed that both oshem 1 and oshem 2 should be classified as a small basic hemolytic peptide. Conclusion : The amino acid sequences of two peptides were highly similar while the primary amino acid sequence analysis revealed W (22th) as the most important mutation. Finally oshem 1 and oshem 2 are the first cytolytic peptides isolated from the Olindias sambaquiensis and should probably represent a novel class of cytolytic peptides. (AU)

Processo FAPESP: 10/50188-8 - Crustáceos decápodes: multidisciplinaridade na caracterização da biodiversidade marinha do estado de São Paulo (taxonomia, espermiotaxonomia, biologia molecular e dinâmica populacional) (Biodiversidade Marinha)
Beneficiário:Fernando Luis Medina Mantelatto
Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático