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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Amperometric glucose biosensor based on layer-by-layer films of microperoxidase-11 and liposome-encapsulated glucose oxidase

Texto completo
Autor(es):
Graca, J. S. [1] ; de Oliveira, R. F. [2] ; de Moraes, M. L. [3] ; Ferreira, M. [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Fed Sao Carlos, UFSCar, BR-18052780 Sorocaba, SP - Brazil
[2] Sao Paulo State Univ, UNESP, Postgrad Program Mat Sci & Technol, BR-17033360 Bauru, SP - Brazil
[3] Univ Fed Sao Paulo, UNIFESP, BR-12231280 Sao Jose Dos Campos, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: BIOELECTROCHEMISTRY; v. 96, p. 37-42, APR 2014.
Citações Web of Science: 33
Resumo

An important step in several bioanalytical applications is the immobilization of biomolecules. Accordingly, this procedure must be carefully chosen to preserve their biological structure and fully explore their properties. For this purpose, we combined the versatility of the layer-by-layer (LbL) method for the immobilization of biomolecules with the protective behavior of liposome-encapsulated systems to fabricate a novel amperometric glucose biosensor. To obtain the biosensing unit, an LbL film of the H2O2 catalyst polypeptide microperoxidase-11 (MP-11) was assembled onto an indium-tin oxide (ITO) electrode followed by the deposition of a liposome-encapsulated glucose oxidase (GOx) layer. The biosensor response toward glucose detection showed a sensitivity of 0.91 +/- 0.09 (mu A/cm(2))/mM and a limit of detection (LOD) of 8.6 +/- 1.1 mu M, demonstrating an improved performance compared to similar biosensors with a single phospholipid-liposome or even containing a non-encapsulated GOx layer. Finally, glucose detection was also performed in a zero-lactose milk sample to demonstrate the potential of the biosensor for food analysis. (C) 2014 Elsevier B.V. All rights reserved. (AU)